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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-diethoxyphenylacetic acid
EC Number:
253-957-3
EC Name:
3,4-diethoxyphenylacetic acid
Cas Number:
38464-04-9
Molecular formula:
C12H16O4
IUPAC Name:
2-(3,4-diethoxyphenyl)acetic acid
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
water

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
The algae were supplied by the „Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen", 37073 Göttingen, Germany.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h

Test conditions

Test temperature:
22 to 23 °C;
pH:
at test start 7.9 to 8.1
at the end of the test 9.2 to 9.5
Nominal and measured concentrations:
100, 31.6, 10.0, 3.2, 1.0 and 0.3 mg test item/L and a control

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
biomass
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 31.6 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 31.6 mg/L
Nominal / measured:
nominal
Basis for effect:
biomass
Details on results:
The influence of Diethoxy acid on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour EyC50 and the 72-hour ErC50 was assessed to be >100 . The 72-hour NOEyC and the 72-hour NOErC were determined to be 31.6 mg test item/L and the associated 72-hour LOEyC and 72-hour LOErC were 100 mg test item/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The influence of Diethoxy acid on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour EyC50 and the 72-hour ErC50 was assessed to be >100 . The 72-hour NOEyC and the 72-hour NOErC were determined to be 31.6 mg test item/L