2,2'-[[5-acetamido-4-[(2-chloro-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, following official guidelines, not GLP compliant and no composition of the tested substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate

EC Number:

222-813-1

EC Name:

2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate

Cas Number:

3618-72-2

Molecular formula:

C23H25BrN6O10

IUPAC Name:

2-{[2-(acetyloxy)ethyl]({4-[ (E)-2-(2-bromo-4,6-dinitrophenyl)diazen-1-yl]-5acetamido-2-methoxyphenyl})amino}ethyl acetate

Test material form:

solid

Study design

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Micro-organisms from a domestic waste water sewage plant (Ara Birsig, Therwil/Switzerland)

Duration of test (contact time):

ca. 28 d

Details on study design:

Apparatus: 50 ml Erlenmeyer flasks loosely covered with an aluminium foil
Test medium: The test medium (30 ml per flask) was prepared according to OECD 301 E (4)
Test concentration: Since the substance was insoluble in water, it was dispersed in the test medium and thereafter filtered through a fluted filter paper. The DOC of the filtrate was then determined. The concentration of the test article corresponded to 28.5 mg DOC/l.
The concentration of the standard (aniline) was 24.8 mg DOC/l. In addition an untretaed inoculated control was run. All samples were run in duplicate

Test conditions: The study was run at 20-25°C protected from light. The inoculated flasks were incubated in a shaking water bath.

Sampling
Per sampling interval, two flasks were taken and analysed for DOC in duplicate. Samples were taken at day 0 (treatment day) 7, 14 and 28 of the incubation period. Water evaporation losses were compensated by adding distilled water.

Samples preparation: Sampls were centrifugated or filtered (pore size: 0.2 micrometres) and thereafter directly analysed. If analyses were performed later 0.05 ml of a 1% HgCl2 solution per 10 ml filtrate were added. Therefater, the samples were stored for 24 hours at 2-4 degrees centigrade or for a longer period of time at -18 degrees.

DOC analyses: DOC analyses were performed with the various filtrates using a Technicon carbon Analyser II,; Technicon Industrial Method N 451-76W) equipped with an automatic Data Processing System.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

ca. 0

Remarks on result:

other: no furtjer details are available

Details on results:

% degradation of aniline in 14 days 98.3
Test substance concentration = not available
aniline concnetration : 32 mg/l

Applicant's summary and conclusion

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The substance was tested for ready biodegradability following OECD 301E. Under the experimental conditions no degradation is observed. The test substance concentration however was not determined and the substance is practically insoluble in water.

Executive summary:

The substance was tested for ready biodegradability following OECD 301E. Under the experimental conditions no degradation is observed. The test substance concentration however was not determined and the substance is practically insoluble in water.