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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Detailed raw data availble, following official guidelines, not GLP compliant

Data source

Reference
Reference Type:
other: original raw data
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate
EC Number:
222-813-1
EC Name:
2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate
Cas Number:
3618-72-2
Molecular formula:
C23H25BrN6O10
IUPAC Name:
2-{[2-(acetyloxy)ethyl]({4-[ (E)-2-(2-bromo-4,6-dinitrophenyl)diazen-1-yl]-5acetamido-2-methoxyphenyl})amino}ethyl acetate
Test material form:
solid
Remarks:
Dark brown

Test solutions

Details on test solutions:
15 min of sonication of diluited solution from a stock solution of 0.1 g/l

Test organisms

Test organisms (species):
Daphnia magna

Study design

Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
261 mg/l CaCO3
Test temperature:
20 °C
pH:
8.3
Dissolved oxygen:
97 %
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
10 Daphnids for each concentration in duplicate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
ca. 4.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Replicate Replicate  Concentration mg/l 24h    48h     % immobility
 1 2  blank  0  0  0  0   0
 29 30  0.9  0  0  0  0   0
31  32  1.9  0  0  0  0   0
 33 34  4.3  0  0  0  0   0
35  36  9.4  0  2  4  5   45
 37 38  21  0  0  3  2   25
39  40  45  0  1  4  3   35
     100 0   0  1 2   20

Applicant's summary and conclusion

Conclusions:
The substance was tested for acute toxicity to aquatic invertebrates following OECD 202. Under the experimental conditions the 48-h EC50 > 100 mg/L.
Executive summary:

The substance was tested for acute toxicity to aquatic invertebrates following OECD 202. Ten animals of Daphnia Magna were tested in duplicate for each concentration. Under the experimental conditions the EC50 > 100 mg/L.