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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on toxicity study of microorganism for the test chemical :
1) luminiscence reduction test was performed for test material on Photobacterium phosphoreum.
2)To evaluate antibacterial activity of test chemical
GLP compliance:
not specified
Test organisms (species):
other: 1) P. phosphoreum 2) Bacillus subtilis
Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min
Test temperature:
1) 15 deg C
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
0.22 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: Luminiscent
Remarks on result:
other: not specified
Dose descriptor:
other: IC50
Effect conc.:
500 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: not specified
Validity criteria fulfilled:
not specified
Conclusions:
The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.
Executive summary:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.

In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.

Description of key information

Toxicity to microorganism:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.

In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.

Key value for chemical safety assessment

Additional information

Toxicity to microorganism:

The effect of test material was evaluated on microorganisms ,Microtox bioassay was performed for the test material onP. phosphoreumbased on the light emission of these bacteria, as a measurement of their metabolic activity. 50% reductionof the light emitted by the bacteria (EC50) is calculated from a concentration response curve by regression analysis. The osmotic pressure of the samples was adjusted by NaCl addition (2%). Toxicity data were based on a 30-min exposure of bacteria to the surfactantsolution at 15 deg C.The effect concentration  EC50 of test material on  P. phosphoreum was evaluated to be 0.22 mg/l.

In another study , the test chemical was used to evaluate minimum inhibition concentration on the test organism Bacillus subtilis after inoculation of test substance , the effect concentration was observed to be 500 mg/l. The effect of test material on bioluminence of bacterial P.phosphorium and growth inhibition of Bacillus subtilis was observed to be 0.22 mg/l and 500 mg/l respectively.