N,N,N-trimethylanilinium chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from authoritative database.

Data source

Reference

Reference Type:

other: authoritative database

Title:

Acute inhalation toxicity of test chemical

Author:

IFA GESTIS

Year:

2018

Bibliographic source:

IFA GESTIS

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Acute inhalation toxicity of the given test chemical in rat.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N,N-trimethylanilinium chloride

EC Number:

205-319-0

EC Name:

N,N,N-trimethylanilinium chloride

Cas Number:

138-24-9

Molecular formula:

C9H14N.Cl

IUPAC Name:

N,N,N-trimethylanilinium chloride

Details on test material:

- IUPAC Name: N,N,N-trimethylanilinium chloride
- Common Name: PTMAC
- InChI: 1S/C9H14N.ClH/c1-10(2,3)9-7-5-4-6-8-9;/h4-8H,1-3H3;1H/q+1;/p-1
- Smiles: [N+](C)(C)(C)c1ccccc1.[ClH-]
- Molecular formula:C9H14ClN
- Molecular weight :171.67 g/mol
- Substance type:Organic
- Physical state:Solid colourless to white inherent odour

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

not specified

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

not specified

Vehicle:

other: aqueous solutions

Remark on MMAD/GSD:

not specified

Details on inhalation exposure:

VEHICLE
- Concentration in vehicle: 550 mg/m³ from a 20% solution while the 2600 mg/m³ and 5600 mg/m³ from a 60% solution.

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

550 mg/m³ from a 20% solution
2600 mg/m³ and 5600 mg/m³ from a 60% solution

No. of animals per sex per dose:

not specified

Control animals:

not specified

Details on study design:

- Necropsy of survivors performed: yes
- Other examinations performed: Animals were observed for mortality and clinical signs.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

LC0

Effect level:

550 mg/m³ air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 2

Sex:

not specified

Dose descriptor:

LC50

Effect level:

1 300 mg/m³ air

Based on:

test mat.

Effect levels 3

Sex:

not specified

Dose descriptor:

LC100

Effect level:

2 600 mg/m³ air

Based on:

test mat.

Exp. duration:

2 h

Effect levels 4

Sex:

not specified

Dose descriptor:

LC100

Effect level:

5 600 mg/m³ air

Based on:

test mat.

Exp. duration:

1 h

Mortality:

550 mg/m3 - Animals were survived.
2600 mg/m³ and 5600 mg/m³ - Caused the death of all the animals.

Clinical signs:

other: The symptoms were attempts to escape, respiratory distress, seizures.

Body weight:

not specified

Gross pathology:

The findings from the necropsy were unspecific (hyperemia of inner organs). Nothing conspicuous was found in the animals which survived.

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The acute inhalation toxicity dose (LC50) was assumed to be 1300 mg/m3, when rats were treated with the given test chemical via inhalation route by aerosols.

Executive summary:

Acute inhalation toxicity study of the given test chemical was conducted in rats at the dose concentration of 550 mg/m³, 2600 mg/m³ and 5600 mg/m³. Rats were exposed to aerosols from aqueous solutions of test chemical.

The given test chemical was exposed as a 20% solution to form 550 mg/m³ and 60% solution to form 2600 mg/m³ and 5600 mg/m³.

The inhalation of a concentration of 550 mg/m³ from a 20% solution was exposed for 4 hours; the inhalation of 2600 mg/m³ exposed for 2 hours and 5600 mg/m³ for 1 hour from a 60% solution.

Animals were observed for mortality and clinical signs.Necropsy of survivors performed. Rats exposed to a concentration of 550 mg/m³ from a 20% solution exposed for 4 hours was survived. While the inhalation of 2600 mg/m³ exposed for 2 hours and 5600 mg/m³ exposed for 1 hour from a 60% solution caused the death of all the animals.

The symptoms were attempts to escape, respiratory distress, seizures. The findings from the necropsy were unspecific (hyperemia of inner organs). Nothing conspicuous was found in the animals which survived.

Under the condition of this, the LC100 value was considered to be 2600 mg/m³ with the exposure time of 2 hours.

Considering the above signs and mortality, the LC50 value was assumed to be 1300 mg/m3, when rats were treated with the given test chemical via inhalation route by aerosols.