p-(dimethoxymethyl)anisole

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study, non-GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German Standard DIN 38412 L9

Principles of method if other than guideline:

Method: other

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-(dimethoxymethyl)anisole

EC Number:

218-577-4

EC Name:

p-(dimethoxymethyl)anisole

Cas Number:

2186-92-7

Molecular formula:

C10H14O3

IUPAC Name:

1-(dimethoxymethyl)-4-methoxybenzene

Details on test material:

- Name of test material (as cited in study report): AIADMA (Anisaldehyddimethylacetal)
- Lot/batch No.: 9/11/88
- Analytical purity: 96.9 % (area)
water solubility: poorly water soluble.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A concentrated stock solution of the test substance in test medium (nominal 500 mg/l) was prepared using Cremophor (100 mg/l) as a solvent. Then, serial dilutions of this stock solution were prepared to obtain the desired test concentrations. The maximum solvent concentration in the test was 50 mg/l.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): Sammlung für Algenkulturen, University of Göttingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

24 °C

pH:

4.5 - 8.3

Nominal and measured concentrations:

Nominal test concentration (mg/l):
5.0, 10.0, 25.0, 50.0, 100.0, 250.0, control, solvent control

Details on test conditions:

TEST SYSTEM
- Initial cells density: about 10000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38 412 L 9


OTHER TEST CONDITIONS
- Photoperiod: continuous white light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Fluorometric measurement of cell density after 0, 24, 48 72 and 96 hours.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

95 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

169 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Reported statistics and error estimates:

EC50 values were determined with appropriate statistical methods (according to Tallarida & Jacob, 1979).

Any other information on results incl. tables

Cell densities after x hours of exposure (*10E-03)

Nominal test  / Exposure time [h]
concentration / 
(mg/l)        /  0 / 24 / 48 / 72  / 96   
Control       / 12 / 20 / 56 / 123 / 263
Solvent       / 12 / 21 / 59 / 134 / 257 
5.0           / 12 / 26 / 62 / 135 / 294
10.0          / 12 / 27 / 65 / 137 / 289
25.0          / 12 / 25 / 65 / 143 / 256
50.0          / 12 / 25 / 60 / 111 / 184
100.0         / 12 / 22 / 49 /  98 / 148
250.0         / 12 / 21 / 27 /  28 /  11

% inhibition of growth rate an biomass
            /   72 h /             96 h
     growth rate u / biomass b / growth rate u / biomass b
Control       -    /   -       /          -    /   -
Solvent       -    /   -       /          -    /   -
5.0           -0.3 /  -6.5     /          -4.4 / -6.6
10.0          -0.9 / -11.1     /          -3.8 / -7.8
25.0          -2.7 / -11.6     /           0.1 / -6.3
50.0           7.8 /   3.1     /          10.9 / 17.8
100.0         13.0 /  21.0     /          18.0 / 31.2
250.0         64.9 /  66.5     /         100.0 / 84.5

EC-values after 72 hours:
EbC10: 95.048 mg/l
EbC50: 168.526 mg/l

EuC10: 94.785 mg/l
EuC50: 173.074 mg/l

EC-values after 96 hours:
EbC10: 77.086 mg/l
EbC50: 132.168 mg/l

EuC10: 62.169 mg/l
EuC50: 85.355 mg/l