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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008-08-22 to 2008-11-28
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD Guideline Study report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylbenzene-1,4-diyl bis{4-[4-(acryloyloxy)butoxy]benzoate}
EC Number:
700-238-1
Cas Number:
132900-75-5
Molecular formula:
C35H36O10
IUPAC Name:
2-methylbenzene-1,4-diyl bis{4-[4-(acryloyloxy)butoxy]benzoate}
Constituent 2
Reference substance name:
4-({4-[4-(Acryloyloxy)butoxy]benzoyl}oxy)-2-methylphenyl 4-[4-(acryloyloxy)butoxy]benzoate
IUPAC Name:
4-({4-[4-(Acryloyloxy)butoxy]benzoyl}oxy)-2-methylphenyl 4-[4-(acryloyloxy)butoxy]benzoate
Details on test material:
Name: Me-3K4A2
Chemical Name: Benzoic acid, 4-[4-[(1-oxo-2-propenyl)oxy]butoxy]-, 2-methyl-1,4-phenylene ester
CAS No.: 132900-75-5
Type: Industrial
Aggregate State at Room Temperature: Powder
Colour: Beige
Density: 0.38 g/cm³
Solubility: In water: < 0.1 g/L
in water: probably stable

Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Duplicate samples from the freshly prepared test media of the only test concentration of nominal 100 mg/L and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of the only test concentration of nominal 100 mg/L and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.

Test solutions

Vehicle:
no
Details on test solutions:
100 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna (Straus), clone 5;From 5 to 22 hours old; Female
The Daphnia introduced in the test were taken from our in house laboratory culture.
The Daphnia were bred in the laboratories of the tesing facility under similar temperature and light conditions as used in the test. The cultivation of the parental Daphnia was performed in reconstituted water of a similar quality with regards to pH, the constituent salts and total hardness as the test medium used in the test. The test organisms were not first brood progeny. Once a week the Daphnia in the stock culture were fed with Tetra Min-extract and at least all other working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of the testing laboratory.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
not reported

Test conditions

Hardness:
2.5 mmol/L (= 250 mg/L) as CaCO3
Test temperature:
20°C at test start and at test end
pH:
7.9 at test start; 7.8 at test end;
Dissolved oxygen:
8.6 to 8.8 mg/L at test start; 8.6 to 8.8 mg/L at test end
Salinity:
not reported
Nominal and measured concentrations:
nominal, substance could not be quantified in the solution
Details on test conditions:
Glass beakers of 100 mL volume containing 80 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions. Each test unit was uniquely identified with serial number, treatment and replicate number.


TEST MEDIUM / WATER PARAMETERS
Reconstituted Water (Elendt M4): In deionised water analytical grade salts and additives will be added at the following nominal concentrations:
Main Compounds Trace Elements Vitamins

CaCl2•2 H2O 293.80 mg/L H3BO3 2860 µg/L Thiamin HCl 75.0 µg/L
MgSO4•7 H2O 123.30 mg/L MnCl2•4 H2O 361 µg/L Cyanocobalamin (B12) 1.0 µg/L
KCl 5.80 mg/L LiCl 306 µg/L Biotin (B6) 0.75 µg/L
NaHCO3 64.80 mg/L RbCl 71 µg/L
Na2SiO3•9 H2O 10.00 mg/L SrCl2•6 H2O 152 µg/L
NaNO3 0.27 mg/L NaBr 16 µg/L
KH2PO4 0.14 mg/L Na2MoO4•2 H2O 63 µg/L
K2HPO4 0.18 mg/L CuCl2•2 H2O 17 µg/L
ZnCl2 13 µg/L
CoCl2•6 H2O 10 µg/L
KI 3.3 µg/L
Na2SeO3 2.2 µg/L
NH4VO3 0.6 µg/L
Na2EDTA•2 H2O 2.5 mg/L
FeSO4•7 H2O 1.0 mg/L



OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h
- Light intensity: 110 - 190 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: no data
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: no data
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: no data
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: no data
Details on results:
After 24 and 48 hours in the control and in the only test concentration of nominal 100 mg test item/L no immobilization of the test animals was observed
Results with reference substance (positive control):
In the most recent test with the reference item potassium dichromate performed in July 2008 the EC50 after 24 hours was determined to be 1.26 mg test item/L indicating that the sensitivity of the Daphnia was consistent with the level proposed by the OECD 202 guideline (EC50-24 h between 0.6 and 2.1 mg potassium dichromate/L).
Reported statistics and error estimates:
The NOEC and LOEC were determined directly from the raw data.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Substance is not harmful to daphnia under the conditions used.
Executive summary:

 

The purpose of this study was to evaluate the influence of the test item Me3K4A2 on the mobility of Daphnia magna according to OECD Guideline 202. Young Daphnia (< 24 hours old) were exposed in a static test for 48 hours to an aqueous test medium containing the test item at the a concentration of 100 mg/l nominal. The test substance is virtually insoluble in water. This limit test was performed in compliance with the test guidelines to demonstrate that the test item has no toxic effect on Daphnia up to at least this concentration.

To avoid physical effects of undissolved test item on Daphnia no concentration above the solubility limit of the test item in test medium was tested. Therefore, a supersaturated stock solution of 100 mg test item/L wa sprepared by suspending 150 mg of test item in 1500 mL of test medium for preparing the test concentration. The stock suspension was stirred for 25.5 hours in the dark to dissolve as much test item as possible. Then, the undissolved test item was separated by filtration and the filtrate was used as test medium. The test concentration was prepared just before introduction of theDaphnia(= start of the test).After 24 hours in the control and in the only test concentration of nominal 100 mg test item/L no immobilization of the test animals was observed.

NOEC [mg test item/L]: ≥ 100(nominal)

LOEC [mg test item/L]: > 100 (nominal)

Also after 48 hours of exposure no immobilization of the test animals was observed in the control and in the only test concentration of nominal 100 mg test item/L.

NOEC [mg test item/L]:≥ 100 (nominal)

LOEC [mg test item/L]: > 100 (nominal)

The toxic effect of the test item Me-3K4A2 to Daphnia magna was assessed in a static limit-test. The 48-hour NEOC was determined to be100 mg test item/L (nominal).