Registration Dossier
Registration Dossier
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EC number: 232-102-8 | CAS number: 7786-67-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Isopulegol
- EC Number:
- 201-940-6
- EC Name:
- Isopulegol
- Cas Number:
- 89-79-2
- Molecular formula:
- C10H18O
- IUPAC Name:
- (1R,2S,5R)-2-Isopropenyl-5-methylcylohexanol
- Details on test material:
- - Name of test substance: L-Isopulegol
- Test substance No.: 10/0358-2
- purity: 99.95 corr. area %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Age (on study day 0): A three day old preculture, prepared in dilution water, was used as inoculum.
- Supplier: Sammlung von Algenkulturen (SAG); Pflanzenphysiologisches Institut der Universität Göttingen; Nikolausberger Weg 18, D-37073 Göttingen
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile headspace flasks, volume: 119 ml, aluminium tops with PTFE seals.
- Initial cells density: 7.085 x 10^3 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: Nutrient medium Z according to LüTTGE et al. (1994)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 24h/d light
- Light intensity and quality: Approximately 60 - 120 μE · m-2 · s-1 (Fluorescent tubes, OSRAM L 36 W/11-865, cool daylight) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 35.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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