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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994-08-29 to 1995-06-09
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
4 April 1992
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted 29 December 1984
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 9.5, 13.3, 18.6, 26, 36.4, 51, 71.4, 100% saturated solution
- Sampling method: samples were analysed in triplicates at 0 and 48 h
- Sample storage conditions before analysis: no data
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 5 g/L (preliminary test) and 0.1 g/L (definitive test) of the test item are stirred for 5 h at 30°C and 1 d at 20°C. Unsolubilised test item is separated by means of centrifugation.
- preliminary test: A concentration range between 100 % and 0.1% was prepared from the saturated stock solution.
- definitive test: Concentrations prepared from saturated stock solution: 100% (64.1 mg/L); 71.4% (40.7 mg/L); 51% (30.1 mg/L); 36.4% (19 mg/L); 26% (15.2 mg/L); 18.6% (9.2 mg/L); 13.3% (7.7 mg/L); 9.5% (7.8 mg/L); 6.8% (
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus
- Source: laboratory's own culture
- Age at study initiation: <24 h
- Feeding during test: no data

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): no data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no
Test temperature:
18-20°C
pH:
- highest initial pH: 7.50
- final pH range: 7.63 - 8.27
Dissolved oxygen:
- final range of oxygen content: 7.4 - 8.1 mg/L
Nominal and measured concentrations:
nominal [% of saturated solution]: 100, 71.4, 51, 36.4, 26, 18.6, 13.3, 9.5, 6.8
measured, initial [mg/L]: 64.1, 40.7, 30.1, 19, 15.2, 9.2, 7.7, 7.8, (measured, final [mg/L]: 59, 38.3, 26.1, 16.6, 12.8, 6.45, (
Details on test conditions:
TEST SYSTEM
- Test vessel: penicllin-type bottles
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: nominal capacity of 120 mL with teflon septa
- Aeration: aeration of solutions until oxygen saturation
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (OECD202), Elendt M7

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation (24 and 48 h)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.4
- Range finding study
- Test concentrations: 0.01 - 100% of saturated solution
- Results used to determine the conditions for the definitive study: results not given in the study report
Reference substance (positive control):
yes
Remarks:
potassium dichromate (results from 1995-05-10)
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
48 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 35 - 100 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
19 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 16 - 24 mg/L
Details on results:
- Other biological observations: none reported
- Mortality of control: 0%
Results with reference substance (positive control):
- Results with reference substance valid? yes
- 24 h EC50: 1.2 mg/L for potassium dichromate (measured 1995-05-10)
Reported statistics and error estimates:
- Final concentrations were found to be within 80% of the initial concentrations with the exception of 18.6%, which was found to be 70% of the initial concentration. However, this result was not considered signifcantly different from the average value of the initial concentration and was therefore included in the interval of confidence.

Initial measured concentration [mg/L]

Number of living Daphnids after 24 h

% immobilised after 24 h

Number of living Daphnids after 48 h

% immobilised after 48 h

0

20

0

20

0

<0.8

19

5

18

10

7.8

19

5

18

10

7.7

19

5

19

5

9.2

19

5

16

20

15.2

19

5

14

30

19

17

15

9

55

30.1

13

35

8

60

40.7

10

50

3

85

64.1

9

55

0

100

Validity criteria fulfilled:
yes
Conclusions:
The 24 h EC50 of TFMEA in Daphnia magna was 48 mg/L (95% CL = 35 - 100 mg/L). The 48 h EC50 was 19 mg/L (95% CL = 16 - 24 mg/L).
Executive summary:

The acute toxicity of TFMEA to Daphnia magna was studied under static conditions according to OECD Guideline 202, adopted 4 April 2008, and EU test method C.2, 29 December 1992. Daphnids were exposed to control, and test chemical at initial concentrations of <0.8, 7.8, 7.7, 9.2, 15.2, 19, 30.1, 40.7, 64.1 mg/L. The 24 h EC50 based on immobilisation was 48 mg/L (95% CL = 35 - 100 mg/L). The 48 h EC50 based on immobilization was 19 mg/L (95% CL = 16 - 24 mg/L).

Results synopsis:

Test Organism Age (e.g. 1stinstar): Daphnia magna Straus, <24 hours old

Test Type: Static  

24 h EC50:  48 mg/L (95% CL = 35 - 100 mg/L)

48 h EC50:  19 mg/L     (95% CL = 16 - 24 mg/L)

Endpoint(s) Effected: mobility

 

Description of key information

48 h EC50=19 mg/L (95% CL = 16 - 24 mg/L); OECD guideline 202/EU method C.2, static

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
19 mg/L

Additional information

One reliable (RL=2), relevant and adequate study on the acute toxicity of TFMEA to aquatic invertebrates is available:

The acute toxicity of TFMEA to Daphnia magna was studied under static conditions according to OECD Guideline 202, adopted 4 April 2008, and EU test method C.2, 29 December 1992. Daphnids were exposed to control, and test chemical at initial concentrations of <0.8, 7.8, 7.7, 9.2, 15.2, 19, 30.1, 40.7, 64.1 mg/L. The 48 h EC50 based on immobilisation was 19 mg/L (95% CL = 16 - 24 mg/L).