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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
other: EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), 4 April 1992
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), 17 July 1992
Deviations:
no
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
not specified
Test organisms (species):
other: no data
Details on inoculum:
- no data
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Nominal and measured concentrations:
3 mg/L
Details on test conditions:
TEST SYSTEM
- Number of culture flasks/concentration: 3 (test substance); 2 (inoculum blank, toxicity control, reference substance)
- Test performed in closed vessels due to significant volatility of test substance: closed bottle test

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes; medium + inoculum
- Abiotic sterile control: no
- Toxicity control: yes; medium + inoculum + test item (3 mg/L) + reference substance (2 mg/L)
- Reference substance: medium + inoculum + reference substance (4 mg/L)
Reference substance (positive control):
no
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The biodegradation reached app. 52% 10 days after the beginning of biodegradation (10-d window). After 21 d a plateau was reached at app. 54.4% degradation.
The reference substance (Sodium benzoate) degraded normally under the test conditions (80.1% after 14 d).
The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28). The test substance showed no inhibitory effects to microorganisms.
Validity criteria fulfilled:
yes
Conclusions:
The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study. According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test item showed no inhibitory effects to microorganisms at 3 mg/L.
Executive summary:

The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), adopted 17 July 1992 and EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), over a period of 28 days. The type of inoculum was not specified in the study report. The biodegradation rate was determined by measurement of O2 consumption. Inoculum blank and procedural/functional control with the reference substance sodium benzoate were performed.

The functional control reached the pass level >60% after 7 d. The biodegradation of the test substance reached 52% 10 days after the beginning of biodegradation (10 d window). After 21 d, a plateau was reached at 54.4% degradation. The pass level of 60% was not reached within the test period.

The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28).

According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test substance showed no inhibitory effects to microorganisms at a concentration of 3 mg/L.

Description of key information

NOEC = 3 mg/L; OECD guideline 301 D, toxicity control; non-GLP

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
3 mg/L

Additional information

One toxicity screening study conducted during the OECD 301 D biodegradation test is available for TFMEA, which is considered reliable with restriction (Klimisch score 2).

The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), adopted 17 July 1992 and EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), over a period of 28 days. The type of inoculum was not specified in the study report. The biodegradation rate was determined by measurement of O2 consumption. Inoculum blank and procedural/functional control with the reference substance sodium benzoate were performed.

The functional control reached the pass level >60% after 7 d. The biodegradation of the test substance reached 52% 10 days after the beginning of biodegradation (10 d window). After 21 d, a plateau was reached at 54.4% degradation. The pass level of 60% was not reached within the test period.

The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28).

According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test substance showed no inhibitory effects to microorganisms at a concentration of 3 mg/L.