Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Mar - May 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.6 - 3.1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

The EC50/48 hours of the test compound to Daphnia magna is expected to be between a dilution of 1 :40 and 1 :80 of the saturated solution. This would be in the range of 2.6 to 3.1 mg/L, based on the initial concentration.

Validity criteria fulfilled:
yes
Conclusions:
Enanthsäureanhydrid or more precisely, its hydrolysis product, is toxic to Daphnia magna, since the EC50 is expected to be between 1 and 10 mg/L.
Executive summary:

The purpose ofthe study was to determine the acute toxicity (immobilization, EC50/48 hours) of Enanthsäureanhydrid with Daphnia magna in order to evaluate the aquatic toxicity. The compound is an intermediate of the synthesis of different therapeutics.

20 juvenile Daphnia magna were used for each test solution of test substance and 20 for the tap water control. The Daphnia were exposed for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solutions a suspension with a nominal loading of 100 mg/L was ultrasonified (30 min) and stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution was further diluted 1 :20, 1 :40, 1 :80, 1: 160 and 1:320. Samples for the concentration analysis by TOC were taken at 0 and 48 hours after start of exposure. The calculated results derived from TOC analysis gave concentrations of 2.9 mg/L (1:320 dilution), 1.3 mg/L (1:160 solution), 2.6 mg/L (1:80 dilution), 3.1 mg/L (1:40 dilution) and 7.1 mg/L (1:20 dilution) for 0 hours. After 48 hours the TOC of the substance vessels could not be differentiated from the TOC of dilution water. Therefore, a definitive concentration over 48 hours could not be given. Since the hydrolytic half-life of the test substance is in the range of 1 to 2 hours, the test substance during the test was most likely the hydrolysis product ZK 11512.

The EC50/48 hours of ZK 56406/ZKl1512 to Daphnia magna is expeeted to be between a dilution of 1:40 and 1:80 of the saturated solution. This would be in the range of 2.6 to 3.1 mg/L, based on the initial concentration.

Description of key information

Enanthsäureanhydrid or more precisely, its hydrolysis product, is toxic to Daphnia magna, since the EC50 is expected to be between 1 and 10 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.1 mg/L

Additional information

"should read: EC50(48h)= 2.6 - 3.1 mg/L"

Categories Display