Methyl non-2-ynoate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Principles of method if other than guideline:

The purpose of this study was to determine the skin irritation and allergic sensitisation potential of Methyl Octine Carbonate in the animal model with guinea pigs according to CTFA safety testing guidelines.
The method was recognised by the international guidelines as the recommended test system - Himalayan white spotted guinea pigs (outbred).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl non-2-ynoate

EC Number:

203-909-2

EC Name:

Methyl non-2-ynoate

Cas Number:

111-80-8

Molecular formula:

C10H16O2

IUPAC Name:

methyl non-2-ynoate

Test material form:

liquid

Test animals

Species:

guinea pig

Strain:

Himalayan

Details on test animals or test system and environmental conditions:

Standard laboratory conditions - air conditioned with 10-15 air changes per hour, temperature around 22 degrees centrigrade.

Test system

Type of coverage:

open

Preparation of test site:

clipped

Vehicle:

not specified

Controls:

yes

Amount / concentration applied:

A single amount of 0.025ml of each test concentration was simultaneously applied to one of the areas in the following concentrationsL
Group 1 (control) - 0%
Group 2 - 3%
Group 3 - 10%
Group 4 - 30%
Group 5 - 100%

Duration of treatment / exposure:

Applications were repeated daily (5 times per week) for 4 weeks using the same skin site. The application site was left uncovered and read 24 hours after each application.

Observation period:

Skin reactions were assessed 24 and 48 hours after the application of the test article.

Number of animals:

30 (15 males, 15 females)

Results and discussion

In vivo

Results

Irritation parameter:

other: Observational results recorded below

Basis:

other: Observation on all animals

Time point:

other: Various time points

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

After repeated applications during 28 successive days except weekends, at the 100% concentration slight irritation was observed in 4 animals and moderate irritation in 3 animals.
After 21 days moderate irritation was observed in 5 animals and slight irritation in 1 animal.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Executive summary:

After repeated applications during 28 successive days except weekends, at the 100% concentration slight irritation was observed in 4 animals and moderate irritation in 3 animals. After 21 days moderate irritation was observed in 5 animals and slight irritation in 1 animal.

Therefore Methyl Octine Carbonate is consdered as a skin irritant, classified as SCI 2.