Reaction mass of (E)-3,7-dimethylocta-2,6-dien-1-yl formate and 3,7-dimethyloct-6-en-1-yl formate

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vivo (LLNA)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No details on the LLNA method, test animals, environmental conditions, only a reference was made. No data whether a positive control substance has been used.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Principles of method if other than guideline:

Kimber, I., Hilton, J., Dearman, R. J., Gerberick, G. F., Ryan, C. A., Basketter, D. A., Scholes, E. W., Ladics, G. S., Loveless, S. E., House, R. V., and Guy, A. (1995) An international evaluation of the murine local lymph node assay and comparison of modified procedures. Toxicology 103, 63-73.

GLP compliance:

no

Type of study:

mouse local lymph node assay (LLNA)

Test material

Test material information

Constituent 1

Reference substance name:

Geranyl formate

EC Number:

203-339-4

EC Name:

Geranyl formate

Cas Number:

105-86-2

Molecular formula:

C11H18O2

IUPAC Name:

(E)-3,7-dimethylocta-2,6-dien-1-yl formate

Constituent 2

Reference substance name:

Citronellyl formate

EC Number:

203-338-9

EC Name:

Citronellyl formate

Cas Number:

105-85-1

Molecular formula:

C11H20O2

IUPAC Name:

3,7-dimethyloct-6-en-1-yl formate

impurity 1

Reference substance name:

Geraniol

EC Number:

203-377-1

EC Name:

Geraniol

Cas Number:

106-24-1

Molecular formula:

C10H18O

IUPAC Name:

(E)-3,7-dimethylocta-2,6-dien-1-ol

impurity 2

Reference substance name:

Rhodinyl formate

EC Number:

205-456-6

EC Name:

Rhodinyl formate

Cas Number:

141-09-3

Molecular formula:

C11H20O2

IUPAC Name:

3,7-dimethyloct-7-en-1-yl formate

Test material form:

liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): Geranyl formate
- Chemical name: trans-formic acid 3,7-dimethyl-octa-2,6-dienyl ester
- The tested substance was an oxidation product of air-exposed geraniol
- The test substance was purified by preparative chromatography

In vivo test system

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals and environmental conditions:

TEST ANIMALS
Three mice were used for each concentration.

ENVIRONMENTAL CONDITIONS
No data

Study design: in vivo (LLNA)

Vehicle:

not specified

Concentration:

0, 5, 10, 20, 40 and 80%

No. of animals per dose:

3

Details on study design:

RANGE FINDING TESTS:
No data.

MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: Local Lymph Node Assay
- Criteria used to consider a positive response: Test materials that at one or more concentrations caused an SI of 3 or higher were considered to be positive in the LLNA. EC3 values (estimated concentration required to produce an SI of 3) were calculated by linear interpolation.

ANIMAL ASSIGNMENT
Five groups of three animals were treated with one test substance concentration per group and one control group of three animals was treated.

TREATMENT PREPARATION AND ADMINISTRATION:
No data, only a reference was made (Kimber, I., Hilton, J., Dearman, R. J., Gerberick, G. F., Ryan, C. A., Basketter, D. A., Scholes, E. W., Ladics, G. S., Loveless, S. E., House, R. V., and Guy, A. (1995) An international evaluation of the murine local lymph node assay and comparison of modified procedures. Toxicology 103, 63-73).

Positive control substance(s):

not specified

Statistics:

Not performed.

Results and discussion

Positive control results:

No data.

In vivo (LLNA)

Results

Results 1

Parameter:

SI

Remarks on result:

other: The SI values calculated for the substance concentrations 5, 10, 20, 40 and 80% were 0.98, 1.1, 2.1, 2.4 and 3.0 respectively.

Results 2

Parameter:

other: disintegrations per minute (DPM)

Remarks on result:

other: The DPM values calculated for the substance concentrations 5, 10, 20, 40 and 80% were 830, 936, 1768, 2022 and 2548 respectively.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (skin sensitising) based on GHS criteria

Conclusions:

In an LLNA skin sensitisation study, Geranyl Formate (air exposed Geraniol) was considered to be a skin sensitiser, as the SI appeared to be ≥ 3 when tested up to and including 80%.

Executive summary:

The sensitizing potency of Geranyl Formate (air exposed Geraniol) was tested in a LLNA assay up to and including concentrations of 80%. Geranyl Formate was considered to be a skin sensitiser, as the SI appeared to be ≥ 3 when tested up to and including 80%.