3-(2-{4-[2-(4-cyanophenyl)vinyl]phenyl}vinyl)benzonitrile

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EC, C.4-D manometric respirometry test

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g., location, sampling depth, contamination history, procedure): The study was performed with aerobic activated sludge from a local wastewater treatment plant treating predominantly domestic wastewater. The sludge was washed by centrifugation, the supernatant liquid phase was decanted and the solid material resuspended in tap water. If necessary, this procedure was repeated. A homogenized aliquot of the final sludge suspension is weighed, thereafter dried and the ratio of wet to dry weight was calculated.
Based on this ratio, calculated amounts of wet sludge were suspended in test water to obtain a concentration equivalent to 4 g dry material (±10 %) per liter. During holding, the sludge was aerated at room temperature until use. Prior to use, the sludge was diluted with test water to a concentration of 1 g per liter (dry weight basis). This diluted activated sludge was used as inoculum to give a final concentration of 30 mg dry material per liter.

Duration of test (contact time):

28 d

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 1 °C, maintained with a built-in thermostat and checked once per week.
- pH: Prior to test start the pH will be measured in all test flasks after the addition of the activated sludge (inoculum), and if necessary adjusted to pH 7.4 ± 0.2 using a diluted hydrochloric acid or sodium hydroxide solution. At the end of incubation the pH will be measured again in all test flasks.

TEST SYSTEM
The test flasks (500-ml Erlenmeyer-flasks, labeled with the RCC project number and all necessary additional information to assure unmistakable identification) will be incubated under continuous stirring in a SAPROMAT D or D12 (Voith GmbH, Heidenheim, Germany). Oxygen consumption will be recorded manually by taking a daily reading at least on each working day.

Reference substance:

other: Sodium benzoate

Preliminary study:

None

Test performance:

None

Parameter:

% degradation (CO2 evolution)

Value:

>= 0 - <= 5

Sampling time:

28 d

Details on results:

0 % degradation after 24 d
0 % degradation after 28 d

Results with reference substance:

None

None

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

Percent degradation was observed as followed:
0 % degradation after 24 d
0 % degradation after 28 d

Executive summary:

The objective of this study is to determine the biodegradability of FAT 60253/A in a 28-day biodegradation test by following the BOD (biochemical oxygen demand) of the test item under defined conditions by means of manometric methods. This test design was selected according to the guidelines since the test item is not completely soluble at a concentration of 100 mg/l (information given by the sponsor). The study design is recognized by the OECD and EEC guidelines and should provide a rational basis to determine the ready biodegradability of the test item under aerobic conditions. Percent degradation was observed as followed:

0 % degradation after 24 d

0 % degradation after 28 d

Description of key information

The Ready biodegradability of FAT 60253/A was investigated in a manometric respirometry test. The degradation of the test material was observed as followes:
0 % degradation after 24 d
0 % degradation after 28 d

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The objective of this study is to determine the biodegradability of FAT 60253/A in a 28-day biodegradation test by following the BOD (biochemical oxygen demand) of the test item under defined conditions by means of manometric methods.

This test design was selected according to the guidelines since the test item is not completely soluble at a concentration of 100 mg/l (information given by the sponsor). The study design is recognized by the OECD and EEC guidelines and should provide a rational basis to determine the ready biodegradability of the test item under aerobic conditions.

Percent degradation was observed as followed:

0 % degradation after 24 d

0 % degradation after 28 d