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EC number: 203-397-0 | CAS number: 106-43-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Principles of method if other than guideline:
- Preincubation method according to Ames, Mutat. Res. 31, 347 (1975), Maron, Mutat. Res. 113, 173 (1983)
The test compound dissolved in 0.05 or 0.1 ml of solvent was supplemented with 0.5 ml of 59mix (metabolie activation method) or O.IM phosphate buffer pH7.4 (direct method) and 0.1 ml of tester strains whieh had been cultured in nutrient broth. The mixture was incubated for 20 min. at 37°C, then rapidly mixed with 2 ml of molten top agar containing 0.05µmol/ml of L-histidine and biotin for the Salmonella test. In the E. coli test 0.05/1 mol/
ml of L-tryptophan was used instead of L-histidine and biotin. Then the top agar mixture was rapidly poured onto a 30 ml of Vogel-Bonner minimal agar plate. All plates were incubated for 48 hours at 37°C and the numbers of revertant colonies were scored. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-chlorotoluene
- EC Number:
- 203-397-0
- EC Name:
- 4-chlorotoluene
- Cas Number:
- 106-43-4
- Molecular formula:
- C7H7Cl
- IUPAC Name:
- 4-chlorotoluene
- Details on test material:
- p-chlorotoluene, purity: 99 %
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA102, TA104, TA1535, TA1537, Escherichia coli WP2uvrA, WP2uvrA/pKM101
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 1) +/-S9: 0.0763, 0.305, 1.22, 4.88, 19.5, 78.1, 313, 1250, 5000 µg/plate;
2) +/-S9: 2.44 (only S.typh strains -S9), 4.88 (only S.typh strains -S9), 9.77, 19.5, 39.1, 78.1, 156, 313, 625 µg/plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: without activation: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, sodium azide, 9-aminoacridine, 2-nitrofluorene, 4-nitroquinoline-N-oxide, bleomycin, pyruvic aldehyde; with activation: 2-amino anthracene
- Details on test system and experimental conditions:
- IUCLID4 Type: Ames test
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA98, TA100, TA102, TA104, TA1535, TA1537, Escherichia coli WP2uvrA, WP2uvrA/pKM101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: from 313 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: Salmonella typhimurium TA98, TA100, TA102, TA104, TA1535, TA1537, Escherichia coli WP2uvrA, WP2uvrA/pKM101
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The positive controls were functional
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
method: an Ames test was conducted using a preincubation protocol in the absence of exogenous metabolic activation, and in the presence of liver S-9.
result: p-chlorotoluene was negative in the test with and without metabolic activation
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