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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
07 Sep 1993 to 01 Dec 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Identification Code : FAT 21036/C
Batch No: EN 410830.32
Appearance: blue solid
Purity: ca. 90 % AS
Solubility (in water): 100 g/L
Storage: room temperature
Stability: 06/98
Analytical monitoring:
yes
Details on sampling:
Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18 °C to - 22 °C until analysis.
Vehicle:
no
Details on test solutions:
Stock solution:
99.9 mg test substance were mixed and made up to 1000 ml with water.
Test organisms (species):
Daphnia magna
Details on test organisms:
Test system: Daphnia magna
Strain: Straus 1820
Source: CIBA-GEIGY Ltd., testing facility
Number of daphnia: 20 per concentration and control. 4 replicates of 5 daphnia each.
Feeding: None during the test
Breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5 1 of reconstituted water (see below) at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.
Pretreatment: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 µm sieve. This operation is repeated 6 hours before the exposure and the young (6-24h of age) are retained for the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Immobilization was recorded after 24 and 48 hours exposure.
Test temperature:
20 ± 1°C
pH:
7.8 - 8
Dissolved oxygen:
99 - 101%
Nominal and measured concentrations:
4.3, 9.4, 20.7, 45.5 and 100 mg test substance/l.
Details on test conditions:
TEST SYSTEM
- Test vessel: Beaker
- Type: closed
- Material: Glass
- Aeration: None
- Volume of solution: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water containing : 65 mg NaHC03, 294 mg CaCl2 2H20, 123 mg MgS04, 7 H20, 6 mg KCl
in 1000 ml bidistilled water. The water was aerated with clean air for at least 24 hours before use.

OTHER TEST CONDITIONS: Lighting: Fluorescent light, 16 hours daily. approx. 1500 lux

EFFECT PARAMETERS MEASURED: Immobilization at 24 and 48 hrs.

VEHICLE CONTROL PERFORMED: no
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
20.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Results:

Chemical analysis of the test preparations at 0 and 48 hours showed equivalence to nominal concentrations obtained at 0 and 48 hours, indicating that the test item was stable under test conditions.

Validity criteria fulfilled:
yes
Remarks:
No immobilization was seen in Control group.
Conclusions:
Exposure of Daphnia magna to FAT 21036/C gave EC50 value of greater than 100 mg/L.
Executive summary:

A study was performed to assess the acute toxicity of FAT 21036/C to Daphnia magna according to EU Method C.2 in a GLP certified laboratory. Following a preliminary range-finding test, groups of twenty daphnids (4 replicates of 5 animals each) were exposed to the aqueous solutions of the test item at concentrations of 4.3, 9.4, 20.7, 45.5 and 100 mg/L for 48 hours at a temperature of approximately 21 °C under static test conditions. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations only. The No Observed Effect Concentration was 20.7 mg/L The immbolisation observed were 10 % at 45.5 and 100 mg/L concentrations. Hence, the 48h EC50 value was >100 mg/L.

Description of key information

In a study performed as per EU Method C.2, groups of twenty daphnids (4 replicates of 5 animals each) were exposed to the aqueous solutions of FAT 21036/C at concentrations of 4.3, 9.4, 20.7, 45.5 and 100 mg/L for 48 hours at a temperature of approximately 21 °C under static test conditions. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations only. The No Observed Effect Concentration was 20.7 mg/L The immbolisation observed were 10 % at 45.5 and 100 mg/L concentrations. Hence, the 48h EC50 value was >100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information