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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Data ia from BAuA report

Data source

Reference
Reference Type:
review article or handbook
Title:
CLH REPORT FOR [3,7-DIMETHYLOCTA-2,6-DIENENITRILE]
Author:
BAuA Federal Institute for Occupational Safety and Health Federal Office for Chemicals Friedrich-Henkel-Weg 1 – 25 D-44149 Dortmund, Germany
Year:
2013
Bibliographic source:
CLH report Proposal for Harmonised Classification and Labelling Based on Regulation (EC) No 1272/2008 (CLP Regulation), Annex VI, Part 2 :Substance Name: 3,7-dimethylocta-2,6-dienenitrile, October 2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
Principles of method if other than guideline:
In vitro chromosome aberration study was performed on 3,7-dimethylocta-2,6-dienenitrile using V79 cells.
GLP compliance:
not specified
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Constituent 1
Chemical structure
Reference substance name:
3,7-dimethylocta-2,6-dienenitrile
EC Number:
225-918-0
EC Name:
3,7-dimethylocta-2,6-dienenitrile
Cas Number:
5146-66-7
Molecular formula:
C10H15N
IUPAC Name:
3,7-dimethylocta-2,6-dienenitrile
Test material form:
other: Liquid
Details on test material:
- Name of test material: 3,7-dimethylocta-2,6-dienenitrile
- Molecular formula: C10H15N
- Molecular weight: 149.236 g/mol
- Smiles notation: N#C\C=C(\CC\C=C(\C)C)C
- InChl : 1S/C10H15N/c1-9(2)5-4-6-10(3)7-8-11/h5,7H,4,6H2,1-3H3/b10-7+
- Substance type: Organic
- Physical state: Liquid

Method

Target gene:
No data
Species / strain
Species / strain / cell type:
other: V79 cells
Details on mammalian cell type (if applicable):
No data
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
No data
Test concentrations with justification for top dose:
0 – 1,000 μg/ml
Vehicle / solvent:
No data available
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
cyclophosphamide
other: EMS
Details on test system and experimental conditions:
No data
Evaluation criteria:
No data
Statistics:
No data

Results and discussion

Test results
Species / strain:
mammalian cell line, other: V79 cells
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Additional information on results:
-The negative controls (vehicle controls) gave frequencies of aberrations within the range expected for the V79 cell line
-Both positive control chemicals, i.e. EMS and cyclophosphamide, led to the expected increase in the number of cells containing structural chromosomal aberrations
-Other confounding effects: The test substance caused a clear, statistically significant and dose-dependent increase in the number of structurally aberrant metaphases incl. and excl.gaps after adding a metabolizing system.
No increase in the frequency of cells containing numerical aberrations was demonstrated.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive with and without

Mutagenic effects were known in chromosome aberration assay with and without metabolic activation study when exposed to V79 cells by 3,7-dimethylocta-2,6-dienenitrile.

Executive summary:

Chromosomal aberration test was performed to determine the mutagenic nature of the test compound 3,7-dimethylocta-2,6-dienenitrile. V79 cells were used for the study and the test chemical was used at dose levels of 0 – 1,000 μg/ml. The test system was used with and without metabolic activation.The test substance caused a clear, statistically significant and dose-dependent increase in the number of structurally aberrant metaphases incl. and excl. gaps after adding a metabolizing system. No increase in the frequency of cells containing numerical aberrations was demonstrated. The negative controls (vehicle controls) gave frequencies of aberrations within the range expected for the V79 cell line. Both positive control chemicals, i.e. EMS and cyclophosphamide, led to the expected increase in the number of cells containing structural chromosomal aberrations. 3,7-Dimethylocta-2,6-dienenitrile was considered to be a chromosome damaging (clastogenic) agent under in vitro conditions in V79 cells.