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Testing m-toluidine towards ready biodegradability in a modified OECD screening test, 64 % to 84 % of the test item were degraded after 28 d, respectively. Furthermore, the pass level is reached within the 10 day window. Thus, the substance can be scored as readily biodegradable.

In an inherent biodegradation test the degradation was 97.7 % after 5 days indicating that the substance is inherently biodegradable. The test was performed with a test design comparable to Zahn-Wellens Test OECD TG 302B.

Anaerobic degradation of m-toluidien could be detected after eight months, within 10 months 73 % of the test item were biodegraded in the sulfate reducing aquifer slurries, but no degradaton could be deteced in the methanogenic aquifer slurries.

m-Toluidine was assessed within the scope of the ICCA/HPV-program, and the test on ready biodegradability conducted by the Chemicals Inspection and Testing Institute, Japan was scored to be reliable for assessment. With a guideline corresponding to the OECD guideline 301C no degradation was observed within 4 weeks related to BOD. Though there is evidence that the test item inhibits the inoculum when tested at higher concentrations. As tests were performed testing 100 mg/L, and there is no information with respect to the performance of toxicity controls, no conclusion can be drawn whether the test item is really not biodegradable.

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