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Ecotoxicological information

Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Principles of method if other than guideline:
Method: 14-day LC50 study was conducted as described by Könemann (Quantitative structure-acitvity relationships in fish toxicity studies. I. Relationship for 50 industrial polluants. Toxicology 19, 209-221, 1981)
GLP compliance:
not specified
Analytical monitoring:
not specified
Details on test solutions:
- stock solutions of the test substance were prepared in 2-propanol
Test organisms (species):
Poecilia reticulata
Details on test organisms:
- Age at study initiation: 2-3 months
Test type:
Water media type:
Limit test:
Total exposure duration:
14 d
25 mg/L CaCO3
Test temperature:
Dissolved oxygen:
>5 mg/L
Details on test conditions:
- 1.5 L vessels, filled with 1L test solution and coverd with glass
- Renewal rate of test solution: daily
- No. of organisms per concentration: 8 fish
- using standard water, prepared according to Alabaster and Abram, 1964
- fish were feed 0.5 h before renewal test solution
- maximal 100 µL of the stock solution were added to 1 L of the standard water

14 d
Dose descriptor:
Effect conc.:
36.3 mg/L
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
not specified
The validity criteria cannot be assessed sufficiently. Nevertheless the test is considered as valid based on the data given.
For the substance a 14d-LC50 of 36.3 mg/L was determined on fish (Poecilia reticulata).
Executive summary:

The short-term toxicity of the substance towards fish was performed in a semi static test system which was in most parts equivalent to the OECD guideline 204 (Fish, prolonged toxicity test), a 14 d-LC50 of 36.31 mg/L was determined.

Description of key information

For the substance a 14d-LC50 of 36.3 mg/L was determined on fish (Poecilia reticulata).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
36.3 mg/L

Additional information

Three studies on acute toxicity towards different fish species are available for m-toluidine.

In a study with m-toluidine a 14d-LC50 of 36.31 mg/l for Poecillia reticulata was determined. The test was performed in a semi static test system according to a method which is in most parts equivalent to the OECD guideline 204 (Fish, prolonged toxicity test) having some deficiencies on documentation of the study design. No information is given, whether test concentrations were checked analytically. But as the substance is known to be stable in aqueous media, is not volatile, not highly sorbing on glas and the test was performed in a semi-static way, constant exposure concentrations over the test duration were assumed.

In another acute test performed with Danio rerio according to the guideline " Lethal effect on Brachydanio rerio LC0, LC50, LC100; 48-96h, UBA-procedural proposal May 1984", a 96h-LC0 of 100mg/L and a 96h-LC100 of 283 mg/L was observed. The geometric mean revealed a 96h-LC50 of 168 mg/L. The difference between measured and nominal concentrations was less than 20 %, therefore the values are given as nominal concentrations.

Further, a lower value on toxicity of m-toluidine to fish was found for the species Oryzias latipes. A 96h-LC50 of 34 mg/l conducted according to OECD TG 203 “Fish, Acute Toxicity Test” is reported. The test was conducted by EA, Japan: "Investigation of the Ecotoxicological Effects of OECD High Production Volume Chemicals", Office of Health Studies, Environmental Health Department, Environment Agency, Japan. The test solutions were prepared in DMSO and it cannot be excluded that observed effects were evoked by the presence of the solvent rather than of the test item, due to deficiencies on documentation. Therefore, the study is not taken into account for assessment.