3',6'-bis(diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03-05-2017 - 03-05-2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

no

Type of method:

HPLC estimation method

Specific details on test material used for the study:

Test Item Name : 3',6'-bis(diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
CAS No. : 509-34-2
Batch No./ Lot no. : KCP/FS/42/17
Date of manufacturing : 2017-01-03
Date of expiry : 2017-12-02
Purity : not available in coa
Stability : Stable in recommended storage conditions
Storage Conditions : Ambient Temp (23 to 27 °C)
Molecular formula : C28-H30-N2-O3
Molecular weight: 442.556

Radiolabelling:

not specified

Test temperature:

25°C

Details on study design: HPLC method:

HPLC Procedure:
The solutions of the test substance and reference substances were prepared in solvent mentioned in 4.3. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were
calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor.

Operating Conditions:
HPLC Model No. : Agilent Technologies,1260 Infinity
Flow Rate : 1.0ml/min
Column : ZORBAX Eclipse Plus C18, 4.6×100mm, 3.5 μm
Mobile phase : ACN: water (55:45)
Temperature : 25°C
Detector : Diode Array Detector @210nm
Injector : Autosampler
Injector volume : 10μl
Data Acquisition software : ChemStationOpen lab Control Panel

Analytical monitoring:

not specified

Key result

Type:

log Koc

Value:

2.68 dimensionless

pH:

7.14

Temp.:

25 °C

Remarks on result:

other: other details not available

Validity criteria fulfilled:

yes

Conclusions:

The Adsorption Coefficient of test substance 3',6'-bis(diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one (CAS No. 509-34-2)was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was found to be 2.682 ± 0.000 at 25°C.

Executive summary:

The adsorption coefficient Koc in soil and in sewage sludge 3',6'-bis(diethylamino)spiro[isobenzofuran- 1(3H),9'-[9H]xanthene]-3-one(CAS No.509-34-2) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference substances were chosen according to functional similarity with the test substance and calibration graph prepared. The reference substances were 4-methylaniline(p-Tolouidine), N-methylaniline, Aniline,Benzoic acid methylester, Xylene, Ethylbenzene, Toluene, Naphthalene having Koc value ranging from 1.8 to 2.75 The Log Koc value was found to be 2.682 ± 0.000at 25°C. This indicates that the substance has moderate sorption to soils and sedimen and slow migration potential to ground water

Description of key information

The Adsorption Coefficient of test substance 3',6'-bis(diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one (CAS No. 509-34-2)was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was found to be 2.682 ± 0.000 at 25°C.

Key value for chemical safety assessment

Koc at 20 °C:

480.839

Additional information

The adsorption coefficient Koc in soil and in sewage sludge 3',6'-bis(diethylamino)spiro[isobenzofuran- 1(3H),9'-[9H]xanthene]-3-one(CAS No.509-34-2) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference substances were chosen according to functional similarity with the test substance and calibration graph prepared. The reference substances were 4-methylaniline(p-Tolouidine), N-methylaniline, Aniline,Benzoic acid methylester, Xylene, Ethylbenzene, Toluene, Naphthalene having Koc value ranging from 1.8 to 2.75 The Log Koc value was found to be 2.682 ± 0.000 (Koc= 480.839) at 25°C. This indicates that the substance has moderate sorption to soils and sedimen and slow migration potential to ground water

[LogKoc: 2.682]