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EC number: 208-096-8 | CAS number: 509-34-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21-03-2017 - 25-03-2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Test Item Name 3',6'-bis(diethylamino)spiro[isobenzofuran- 1(3H),9'-[9H]xanthene]-3-one
- CAS No. 509-34-2
- Batch No. KCP/FS/42/17
- Physical Appearance pinkish red powder
- Date of manufacturing 2017-01-03
- Date of expiry 2017-12-02
- Purity not available in coa
- Stability Stable in recommended storage conditions
- Storage Conditions 1. Ambient Temp (23 to 27 °C) - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 2 hours continuous stirring for achieving test concentrations of 6.25mg/L, 12.5mg/L, 25mg/L, 50mg/L,100mg/L ,respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish
- Strain:Danio rerio
- Source: Koyal aqurium,Nagpur.
- Age at study initiation (mean and range, SD):-
- Length at study initiation (length definition, mean, range and SD): average 1.79 cm
- Weight at study initiation (mean and range, SD): 0.1204g (average)
- Method of breeding: -
- Feeding during test: Withdrawal of food 1 day before and during exposure
- Food type:Standard brand feed,
- Amount:-
- Frequency: once daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 148.5 mg of CaCO3
- Test temperature:
- 23 °C
- pH:
- 7.03
- Dissolved oxygen:
- 7.80 mg/L
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- 6.25mg/L,12.5mg/L, 25mg/L, 50mg/L and 100mg/L
- Details on test conditions:
- Photoperiod: 16 hours light and 8 hours dark
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates: Test started at : 13-03-2017
Test Exposure date: 21-03-2017
Date of experimental termination : 25-03-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
1
1
2
2
100
4
4
-
-
-
-
4
4
4
4
4
4
4
4
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Symptom (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
50
4
4
NS4
NS4
NS4
NS4
NS4
NS4
M4
M4
M3
M3
M2
M2
100
4
4
NS4
NS4
M4
M4
-
-
-
-
-
-
-
-
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
8.04
8.04
8.12
8.15
8.23
8.25
8.32
8.35
6.25
4
4
7.41
7.42
8.12
8.12
8.21
8.22
8.36
8.36
12.5
4
4
7.50
7.50
8.15
8.16
8.29
8.30
8.39
8.38
25
4
4
7.55
7.56
8.24
8.24
8.31
8.31
8.40
8.41
50
4
4
7.61
7.61
8.29
8.30
8.35
8.36
8.45
8.42
100
4
4
-
-
-
-
-
-
-
-
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.22
7.21
7.01
7.01
6.90
6.90
6.80
6.82
6.25
4
4
7.09
7.08
7.34
7.34
7.21
7.21
7.00
7.01
12.5
4
4
6.88
6.89
7.32
7.32
7.14
7.15
7.02
7.03
25
4
4
6.82
6.83
7.31
7.31
7.17
7.17
7.01
7.00
50
4
4
6.98
6.95
7.29
7.29
7.21
7.22
7.03
7.05
100
4
4
-
-
-
-
-
-
-
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.509-34-2 on Zebra Fish (Danio rerio) was found to be 50 mg/L.
- Executive summary:
A 96 h study was conducted for CAS No. 509-34-2 on Zebra fish(Danio rerio), at a test concentrations of 6.25mg/L,12.5mg/L, 25mg/L, 50mg/L and 100mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 50 mg/L.
LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L
LC50 (96 hours) Experimental = 50 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L
This value indicates that the substance 3',6'-bis(diethylamino)-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one is toxic to fish and can be classified as Aquatic chronic category 3.
Reference
Description of key information
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.509-34-2 on Zebra Fish (Danio rerio) was found to be 50 mg/L. This value indicates that the substance 3',6'-bis(diethylamino)-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one is toxic to fish and can be classified as Aquatic chronic category 3.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 50 mg/L
Additional information
Various studies, considering its adequacy as one key and other supporting, were reviewed for the endpoint short term toxicity to fish of the substance 3',6'-bis(diethylamino)-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one and same are summarized as below:
In a key study, 96 h study was conducted by SSS for CAS No. 509-34-2 on Zebra fish (Danio rerio), at a test concentrations of 6.25mg/L,12.5mg/L, 25mg/L, 50mg/L and 100mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 50 mg/L.
LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L
LC50 (96 hours) Experimental = 50 mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L
This value indicates that the substance 3',6'-bis(diethylamino)-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one is toxic to fish and can be classified as Aquatic chronic category 3.
In supporting study for read across Rhodamine B (Cas no. 81-88-9) from Experimental data (J. Toxicol. Sci. 7: 193; 1982) indicated that fish toxicity study was conducted for 48 hrs for evaluating the lethal concentration of test substance Rhodamine B on fish Oryzias latipes on the basis of mortality effect. During experiment the lethal concentration (LC50) for test substance Rhodamine B was found to be 17 mg/l for 24 hrs. and 12 mg/l for 48 hrs. This LC50 value indicate that the test substance Rhodamine B is toxic to fish and classified in aquatic chronic 3 category as per the CLP criteria.
For the same read across substance, i.e Rhodamine B (Cas no. 81-88-9); Fish toxicity study (Japan chemicals collborative knowledge database (J-check); 2017) was conducted for 48 hrs for evaluating the lethal concentration of test substance Rhodamine B on fish Oryzias latipes on the basis of mortality effect. During experiment the lethal concentration (LC50) for test substance Rhodamine B was determined to be 33.9 mg/l. This LC50 value indicate that the test substance Rhodamine B is toxic to fish and classified in aquatic chronic 3 category as per the CLP criteria.
Both studies for read across chemical give assistance to experimental result of target chemical.
Thus, based on experimental study conducted by SSS with EC50= 50 mg/l, it can be concluded that the target chemical 3',6'-Bis(N,N-diethylamino)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one (CAS no. 509-34-2) is toxic to fish and thus can be classified in Aquatic Chronic 3 category as per the CLP criteria.
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