3-hydroxy-3'-nitro-2-naphthanilide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-3'-nitro-2-naphthanilide

EC Number:

205-209-2

EC Name:

3-hydroxy-3'-nitro-2-naphthanilide

Cas Number:

135-65-9

Molecular formula:

C17H12N2O4

IUPAC Name:

3-hydroxy-N-(3-nitrophenyl)-2-naphthamide

Test material form:

solid

Details on test material:

purity: 99.7%
Product number HKAG 155

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

sludge provided from the waste water treatment plant Frankfurt am Main
was washed two times and centifuged and resuspended in demineralized water

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

25.87 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

The test was performed in a room with a temperature of 22 +- 2°C..
Reaction vessel: 5 l glass bottle with magnetic stirrer.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0.1

Sampling time:

2 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

16 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

After 28 days the reference substance sodium benzoate showed a biodegradation of 72.8% under the conditions applied.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item is not readily biodegradable

Executive summary:

The substance was tested for ready biodegradation with the "Sturm Test" according to OECD guideline 301B. After 28 days the substance showed no CO2 evolution under the conditions applied,

whereas the reference substance has a biodegradation of 72.8%. Therefore, the test item has considered to be not readily biodegradable.