Cyclohexyl phenyl ketone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984 followed

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992 followed

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): TKA 40143
- Physical state: solid
- Analytical purity: 88.8%
- Lot/batch No.: PNr. 5021
- Expiration date of the lot/batch: December 01, 1997
- Storage condition of test material: at room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution was prepared at a nominal concentration of 100 mg/L: 503.4 mg of test substance was dispersed in 5 liters of test medium using ultra-sonic treatment and intensive stirring. Temperature of the medium had increased to 35°C during preparation. The final stock solution was a homogeneous suspension. This stock was used to prepare the different test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): < 24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

218 mg CaCO3/L

Test temperature:

20.2-21.0ºC

pH:

Controls: 8.0 - 8.1
100 mg/L: 7.8 - 8.0

Dissolved oxygen:

Controls 9.6 - 9.4 mg O2 /L
100 mg/L: 9.0 - 9.0 mg 02 /L

Nominal and measured concentrations:

Nominal: 0.1, 1.0, 10, 100 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO-medium

EFFECT PARAMETERS MEASURED: Mobility observed at 24 and 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

At 10 and 100 mg/L after 24 hours, all daphnids were found trapped at the surface of the test solution, they were re-immersed and then observed for mobility.

At 1.0 mg/L after 24 hours, a total of 3 daphnids were found trapped at the surface of the test solution, they were re-immersed and then observed for mobility.

At 100 mg/L, precipitation of the test substance was observed.

There was no immobility in any of the control samples.