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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from NTIS study report

Data source

Reference
Reference Type:
secondary source
Title:
Summary of toxicity studies for 4-Methoxyphenyl-Acetic Acid (MPAA)
Author:
National Technical Information Service
Year:
1991
Bibliographic source:
National Technical Information Service, OTS0530656-1 (1991)

Materials and methods

Principles of method if other than guideline:
Acute dermal toxicity studies of (4-methoxyphenyl) acetic acid were carried out on ten new Zealand rabbits(Male and female) under occlusive wrapping to determine the LD50.
GLP compliance:
not specified
Test type:
other: Acute dermal
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-methoxyphenylacetic acid
EC Number:
203-166-4
EC Name:
4-methoxyphenylacetic acid
Cas Number:
104-01-8
Molecular formula:
C9H10O3
IUPAC Name:
(4-methoxyphenyl)acetic acid
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): 4-methoxyphenyl acetic acid (MPAA)
- Molecular formula (if other than submission substance): C9-H10-O3
- Molecular weight (if other than submission substance): 166.175
- Smiles notation (if other than submission substance): c1(ccc(OC)cc1)CC(O)=O
- InChl (if other than submission substance): 1S/C9H10O3/c1-12-8-4-2-7(3-5-8)6-9(10)11/h2-5H, 6H2, 1H3, (H,10,11)
- Substance type: Organic
- Physical state: Solid
Purity-100%
Specific details on test material used for the study:
- Name of test material (as cited in study report):(4-methoxyphenyl) acetic acid
- Molecular formula: C9H10O3
- Molecular weight: 166.175 g/mole
- Substance type: Organic

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals or test system and environmental conditions:
No details available

Administration / exposure

Type of coverage:
occlusive
Vehicle:
not specified
Details on dermal exposure:
TEST SITE
- Area of exposure:no data
- % coverage:no data
- Type of wrap if used:occlusive wrapping with absorbent pad
Duration of exposure:
24 hrs
Doses:
2000 mg/kg
No. of animals per sex per dose:
5 male, 5 female
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: hourly for first four hours and then twice daily.
- Necropsy of survivors performed: yes
- Frequency of observations and weighing: Twice daily
- Other examinations performed :Mortality, clinical signs, skin Condition and gross pathology were examined.
Statistics:
no data

Results and discussion

Preliminary study:
No data available
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No effect on survival observed
Mortality:
No mortality were observed in treated rabbits at 2000 mg/kg bw
Clinical signs:
Systemic clinical signs were minimal and consisted of abnormal stools in three animals during the first or second day of study
Body weight:
Weight gained during study were observed in all the treated rabbits at 2000 mg/kg bw
Gross pathology:
No macroscopic adverse effects were observed on necropcies.
Other findings:
Skin reaction: Erythema and edema were observed in 3 animals which are free of dermal irritation 5 days of treatment.

Applicant's summary and conclusion

Interpretation of results:
not classified
Conclusions:
LD50 was considered to be > 2000 mg/kg bw when New Zealand White male and female rabbits were treated with (4-methoxyphenyl) acetic acid under an occlusive wrapping for 24 hours.
Executive summary:

In a acute dermal toxicity study, New Zealand White 5 male and 5 female rabbits were treated with 4-methoxyphenylacetic acid in the concentration of 2000 mg/kg under an occlusive wrapping for 24 hours. No mortality were observed in treated rabbits at 2000 mg/kg bw. Systemic clinical signs were minimal and consisted of abnormal stools in three animals during the first or second day of study and Weight gain during study were observed in all the treated rabbits at 2000 mg/kg bw. Erythema and edema were observed in 3 animals which are free of dermal irritation 5 days of treatment and no macroscopic adverse effects were observed in necropsies. Therefore, LD50 was considered to be > 2000 mg/kg bw when New Zealand White male and female rabbits were treated with 4-methoxyphenylacetic acid under an occlusive wrapping for 24 hours.

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