Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-166-4 | CAS number: 104-01-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Skin Irritation:
The minimum mean dermal irritation score at any observation was 0.0 (Maximum possible score is 8.0).No animal showed any indication of dermal irritation.
Thus, it was considered that 4 methoxy phenylacetic acid (MPAA) was not irritating to skin.
Eye Irritation:
The maximum mean irritation score was 71.2 (of 110 possible maximum) which occurred on day 3.
4-MethoxyphenyIacetic Acid (MPAA) was considered to be a very severe irritant to the eye of albino rabbits under the conditions of these studies causing effects which were not reversed within 21 days of treatment. Immediate washing of the eyes resulted in reduced irritation and more rapid recovery.
4-MethoxyphenyIacetic Acid (MPAA) may be classified under the category of eye irritant.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from NTIS study report.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Skin irritation study was carried out to test the effects of 4 methoxy phenylacetic acid (MPAA) on New Zealand rabbits at concentration of 0.5gms.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):4 methoxy phenylacetic acid (MPAA)
- Molecular formula : C9H10O3
- Molecular weight : 166.175g/mol
- Smiles notation: c1(ccc(OC)cc1)CC(O)=O
- InChl : 1S/C9H10O3/c1-12-8-4-2-7(3-5-8)6-9(10)11/h2-5H, 6H2, 1H3, (H,10,11)
- Substance type: Organic
- Physical state: Solid - Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- No data available
- Type of coverage:
- other: Gauze patch and adhesive tape
- Preparation of test site:
- clipped
- Vehicle:
- water
- Controls:
- not specified
- Amount / concentration applied:
- 0.5gms
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 1 hrs after removal of cover and 1,2,3 days after removal
- Number of animals:
- 6 animals
- Details on study design:
- TEST SITE
- Area of exposure: 6 square cms of clipped skin
- Type of wrap if used: Gauze patch
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Skin wiped free of excess test material with absorbant pad
- Time after start of exposure: 4 hours
SCORING SYSTEM: Draize scoring system approx 1 hr after removal of cover and 1,2 3 days after removal. - Irritation parameter:
- other: maximum mean dermal irritation score
- Basis:
- mean
- Time point:
- other: 1 hr,1,2 and 3 days
- Score:
- 0
- Max. score:
- 8
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- not irritating to skin of rabbits
- Interpretation of results:
- other: not irritating
- Conclusions:
- 4 methoxy phenylacetic acid (MPAA) was considered to be not irritating under test conditions of this study.
- Executive summary:
A skin irritation study was carried out to test the effects of 4 methoxyphenyl acetic acid on New Zealand rabbits. Six New Zealad White rabbits were treated with 0.5 gm of test substance moistened with water.The Area of exposure was 6 square cm of clipped skin and the wrap used was Gauze patch. The cover was removed after 4 hours of exposure and the skin was wiped free of excess test material with absorbant pad.The skin reactions in the rabbits were observed and evaluated according to Draize method after 1 hr of removal of cover and 1, 2,3 days after removal . The minimum mean dermal irritation score at any observation was 0.0 (Maximum possible score is 8.0). No animal showed any indication of dermal irritation.
Thus, it was considered that 4 methoxy phenylacetic acid (MPAA) was not irritating to skin.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from NTIS study report.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The eye irritation potential of 4-methoxyphenylacetic acid was assessed in rabbits according to the Draize method.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-methoxyphenylacetic acid
- Molecular formula : C9H10O3
- Molecular weight : 166.175g/mol
- Smiles notation : c1(ccc(OC)cc1)CC(O)=O
- InChl : 1S/C9H10O3/c1-12-8-4-2-7(3-5-8)6-9(10)11/h2-5H, 6H2, 1H3, (H,10,11)
- Substance type: Organic
- Physical state: Solid - Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- No data available
- Vehicle:
- physiological saline
- Controls:
- not specified
- Amount / concentration applied:
- 0.1gm
- Duration of treatment / exposure:
- 21 days
- Observation period (in vivo):
- 1hr ,1,2, 3,4 ,7,14and 21 days
- Duration of post- treatment incubation (in vitro):
- no data available
- Number of animals or in vitro replicates:
- 9 animals (one animal and 8 additional animals)
- Details on study design:
- TEST SITE: Right eye
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Yes with room temperature saline solution 30 seconds after application of test material. - Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 3 days
- Score:
- 71.2
- Max. score:
- 110
- Reversibility:
- not reversible
- Remarks on result:
- probability of severe irritation
- Irritant / corrosive response data:
- Discharge ,swelling ,redness between one hr and 3 days Cornal opacity, iritis and conjunctival irritation.
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- 4-MethoxyphenyIacetic Acid (MPAA) was considered to be a very severe irritant to the eye of New Zealand White rabbits under the conditions of these studies causing effects which were not reversed within 21 days of treatment. Immediate washing of the eyes resulted in reduced irritation and more rapid recovery.
4-MethoxyphenyIacetic Acid (MPAA) may be classified under the category 2 (irritating to eyes). - Executive summary:
The eye irritation potential of 4-methoxyphenylacetic acid was assessed in rabbits according to the Draize method. The test was conducted on New Zealand White rabbits.
Test is first performed on one rabbit with 0.1gm of test substance, the treated eye showed moderate irritation, Thereafter 8 additional animals were tested and subjected to rinsing with normal saline after 30 seconds of administration .Ocular lesions were scored approx 1 hr, 1 day upto 21 days. Even 21 days after treatment, un-rinsed eyes in 5/6 treated animals displayed corneaI opacity, iritis and conjunctival irritation. The maximum mean irritation score was 71.2 (of 110 possible maximum) which occurred on day 3, the rinsed eyes showed milder effects; however, serious adverse effects persisted to day 21. 4-MethoxyphenyIacetic Acid (MPAA) was considered to be a very severe irritant to the eye of albino rabbits under the conditions of these studies causing effects which were not reversed within 21 days of treatment. Immediate washing of the eyes resulted in reduced irritation and more rapid recovery.
4-MethoxyphenyIacetic Acid (MPAA) may be classified under the category 2 (irritating to eyes).
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin Irritation:
For evaluation of dermal irritation potential of 4-MethoxyphenyIacetic Acid (MPAA),4 studies of target chemical along with read across substance are summarized as follows:
Study 1:
A skin irritation study was carried out by NTIS (1991), to test the effects of 4 methoxyphenyl acetic acid on New Zealand rabbits. Six New Zealand White rabbits were treated with 0.5 gm of test substance moistened with water. The Area of exposure was 6 square cm of clipped skin and the wrap used was Gauze patch. The cover was removed after 4 hours of exposure and the skin was wiped free of excess test material with absorbant pad. The skin reactions in the rabbits were observed and evaluated according to Draize method after 1 hr of removal of cover and 1, 2, 3 days after removal . The minimum mean dermal irritation score at any observation was 0.0 (Maximum possible score is 8.0).No animal showed any indication of dermal irritation.
Thus, it was considered that 4 methoxy phenylacetic acid (MPAA) was not irritating to skin.
Studies 2, 3 are estimated data for 4-methoxy phenylacetic acid (MPAA)
Study 2:
The dermal irritation potential of 4-methoxyphenylacetic acid (MPAA) was predicted using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances.
4 -methoxy phenylacetic acid(MPAA) was estimated to be not irritating to the skin of New Zealand White rabbits.
On the basis of the estimated result, 4 -methoxy phenylacetic acid (MPAA) can be considered to be not irritating to skin and can be classified under the category "Not Classified" as per CLP regulation.
Study 3:
Skin irritation effects were estimated by four different models i.e, Battery, leadscope, SciQSAR and CASE Ultra used within Danish QSAR database for chemical 4-methoxy phenylacetic acid.
Based on estimation, no severe skin irritation effect were known when test chemical, 4 -methoxy phenylacetic acid was exposed to rabbit skin.
The estimated results of Studies 2, 3 are in agreement with the experimental data (Study 1)
Study 4:
A pre-screen test for a human maximization study was conducted (Food and Chemical Toxicology 50 (2012) S134–S139) to evaluate the irritation potential of the similar substance 105-13-5 (4-methoxybenzyl alcohol).
5% anisyl alcohol in petrolatum (3450 microgram/cm2) was applied under occlusion to the normal skin of seven subjects for 48 h and the effects were observed.
No irritation was observed in all the volunteers after 48 hours.
Hence, 4 -methoxybenzyl alcohol can be considered as not irritating to skin.
The read across study also supports to the possibility of 4 methoxy phenylacetic acid (MPAA) being not irritating to skin.
Therefore, by applying the weight of evidence approach, 4 methoxyphenylacetic acid (MPAA) can be considered to be not irritating to skin. It can be classified under the category "Not Classified" as per CLP regulation.
Eye Irritation:
For evaluation of ocular irritation potential of 4-MethoxyphenyIacetic Acid (MPAA), 3 studies of target chemical along with relevant read across substances are summarized as follows:
Study 1:
The eye irritation potential of 4-methoxyphenylacetic acid was assessed (NTIS;1991) in rabbits according to the Draize method. The test was conducted on New Zealand White rabbits.
Test is first performed on one rabbit with 0.1gm of test substance, the treated eye showed moderate irritation, Thereafter 8 additional animals were tested and subjected to rinsing with normal saline after 30 seconds of administration .Ocular lesions were scored approx 1 hr 1 day upto 21 days. Even 21 days after treatment, un-rinsed eyes in 5/6 treated animals displayed corneaI opacity, iritis and conjunctival irritation. The maximum mean irritation score was 71.2 (of 110 possible maximum) which occurred on day 3, the rinsed eyes showed milder effects; however, serious adverse effects persisted to day 21. 4-MethoxyphenyIacetic Acid (MPAA) was considered to be a very severe irritant to the eye of albino rabbits under the conditions of these studies causing effects which were not reversed within 21 days of treatment. Immediate washing of the eyes resulted in reduced irritation and more rapid recovery.
4-MethoxyphenyIacetic Acid (MPAA) may be classified under the category of eye irritant.
Studies 2, 3 are the experimental data for the relevant read across substances.
Study 2:
A rabbit eye irritation study was performed (SAX (Sax's Handbook of Dangerous Industrial Materials), 2007) to assess the irritation potential of the similar substance 111-27-3 (hexan-1-ol).
250 g of hexan-1-ol instilled into rabbit eye and effects were observed till 72 hours and reactions were scored. The maximum score was 10. Scores over 5 were considered to cause severe eye injury.
Hexan-1-ol was graded 9/10 after 72 hours. Based on the scores, Hexan-1-ol can be considered as a severe eye irritant with a possibility to cause injury to rabbit eyes.
Study 3
Acute toxicity studies were carried out (American Industrial Hygiene Association Journal, (1973), 34:11, 493-499) to estimate the toxicity of the similar substance 112-70-9 (tridecyl alcohol).
A single application of 0.1 ml of undiluted tridecyl alcohol was made into the conjunctival sac of the left eye of each of six rabbits. The untreated eye served as the control. The treated eye was closed for 30 seconds and eyes were not washed. Observations for irritancy and possible systemic toxicity were made at 1, 4, and 24 hours and at 2, 3, 4, and 7 days. Where positive effects persisted, observations were continued initially and at termination the eyes were examined following instillation of 2 % sodium fluorescein solution to detect any corneal lesions if not otherwise visible. Grading scale is slight-moderate-marked and severe. Signs of irritation exposure were graded and scored according to the method of Draize.
Tridecyl alcohol caused little irritation in most of the animals, but persistent corneal opacity in one caused the classification as moderate. Tridecyl alcohol can be considered as moderately irritating to eyes.
The read across studies also supports to the possibility of 4 methoxy phenylacetic acid (MPAA) being irritating to eyes.
Therefore, by applying the weight of evidence approach, 4 methoxy phenylacetic acid (MPAA) can be considered to be irritating to eyes. It can be classified under the category "Category 2 (irritating to eyes)" as per CLP regulation.
Justification for classification or non-classification
Available studies for 4 methoxyphenylacetic acid (MPAA) indicate that it is not irritating to skin but irritating to eyes.
4-methoxyphenylacetic acid (MPAA) can be classified under the category "Not Classified" for Skin Irritation, and “Category 2 (irritating to eyes)" for Eye Irritation as per CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
