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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Title:
No information
Author:
Mortelmans K et al
Year:
1986
Bibliographic source:
Environ Mutagen 8:1-119 (1986)

Materials and methods

Test guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzoin
EC Number:
204-331-3
EC Name:
Benzoin
Cas Number:
119-53-9
Molecular formula:
C14H12O2
IUPAC Name:
2-hydroxy-1,2-diphenylethanone

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Metabolic activation system:
rat and hamster S9 fraction
Test concentrations with justification for top dose:
33 to 3333 microgram/plate

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Additional information on results:
Benzoin was found to be negative when tested for mutagenicity using the
Salmonella/microsome preincubation assay, using the standard protocol
approved by the National Toxicology Program (NTP). Benzoin was tested in
as many as 6 Salmonella typhimurium strains (TA1535, TA1537, TA 1538, TA97, TA98,
and TA100) in the presence and absence of rat and hamster liver S-9, at
doses of 0.033, 0.0333, 0.100, 0.333, 0.3333, 0.667, 1.000, and 3.3333
mg/plate. The highest ineffective dose tested in any S. typhimurium strain
was 3.3333 mg/plate. Precipitate was observed in most cultures at the high
dose and also some clearing of the background bacterial lawn was also
seen. [Mortelmans K et al; Environ Mutagen 8:1-119 (1986)]
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion