3,5-diaminobenzoic acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Study of Photobacterium phosphoreum at 5, 15 or 30 min for exposure to chemical as 50% reduction of the light emission of the test bacteria in a given time.

GLP compliance:

no

Specific details on test material used for the study:

- EC name:3,5 Diaminobenzoic acid
- Name of test material (as cited in study report): 3,5-diaminobenzoic acid
- Molecular formula (if other than submission substance): C7H5NO4
- Molecular weight (if other than submission substance): 167.1195 g/mol
- Smiles notation (if other than submission substance): c1(cc(ccc1)[N+](=O)[O-])C(=O)O
- InChI: 1S/C7H5NO4/c9-7(10)5-2-1-3-6(4-5)8(11)12/h1-4H,(H,9,10)
- Substance type: Organic
- Physical state: Solid
- Analytical purity: 98%

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Photobacterium phosphoreum

Details on inoculum:

Strain : NRRL-B-11177

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

30 min

Remarks on exposure duration:

Experiment conduct at 5 min,15 min and 30 min

Hardness:

No data

Test temperature:

15 deg.C

pH:

>5 to <9

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

31.8 mg/l

Details on test conditions:

No data

Reference substance (positive control):

not specified

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

31.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: 50% reduction of the light emission of the test bacteria in a given time

Validity criteria fulfilled:

not specified

Conclusions:

The effect concentration i.e EC50 value for toxicity to microorganism species Photobacterium phosphoreum (Strain : NRRL-B-11177) for test chemical 3, 5 diaminobenzoic (535-87-5) acid was observed to be 31.8 mg/l at 30 min.exposure period.

Executive summary:

Toxicity study of test chemical 3,5 Diaminobenzoic acid (Cas no. 535-87-5) for the luminescent marine bacterium Photobacterium phosphoreum(Strain : NRRL-B-11177)was determine by MicrotoxTM test.

Study conducted for 5, 15 or 30 min exposure and this test was determined at a temperature of 15 deg.C. and pH range >5 to <9 was used. Study was to calculate the effective concentration EC50 value of photobacterium phosphorum 50% reduction of the light emission.

At 30 min. exposure period EC50 value for toxicity to microorganism of test chemical 3, 5 diaminobenzoic (Cas no.535-87-5) acid was observed to be 31.8 mg/l.

Description of key information

Toxicity study of test chemical 3,5 Diaminobenzoic acid (Cas no. 535-87-5) for the luminescent marine bacterium Photobacterium phosphoreum(Strain : NRRL-B-11177)was determine by MicrotoxTM test.

Study conducted for 5, 15 or 30 min exposure and this test was determined at a temperature of 15 deg.C. and pH range >5 to <9 was used. Study was to calculate the effective concentration EC50 value of photobacterium phosphorum 50% reduction of the light emission.

At 30 min. exposure period EC50 value for toxicity to microorganism of test chemical 3, 5 diaminobenzoic (Cas no.535-87-5) acid was observed to be 31.8 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

31.8 mg/L

Additional information

Three studies including experimental studies from peer reviewed journal and predicted data from validated models for micro organism endpoint of test chemical 3,5-Diaminobenzoic acid (CAS no. 535 -87 -5) with read across chemical which is closest to target using log kow as the primary descriptor were reviewed as follows:

First study from the peer reviewed journal (Water Poll. Res. J. Canada; 1991 Volume 26, No. 3, 361-431) indicate toxicity study of test chemical 3,5 Diaminobenzoic acid (Cas no. 535-87-5) for the luminescent marine bacterium Photobacterium phosphoreum(Strain : NRRL-B-11177)was determine by MicrotoxTM test.Study conducted for 5, 15 or 30 min exposure and this test was determined at a temperature of 15 deg.C. and pH range >5 to <9 was used. Study was to calculate the effective concentration EC50 value of photobacterium phosphorum 50% reduction of the light emission. At 30 min. exposure period EC50 value for toxicity to microorganism of test chemical 3, 5 diaminobenzoic (Cas no.535-87-5) acid was observed to be 31.8 mg/l.

Above Experimental data for target was supported by predicted report using QSAR toolbox version 3.4. The prediction indicate the toxicity to micro organism of the substance 3,5 Diaminobenzoic acid (Cas no. 535-87-5) to Tetrahymena pyriformis based on the effects observed in a static freshwater system during a 48 hr exposure. The Inhibition growth concentration (IGC50) for the substance 3,5 Diaminobenzoic acid is estimated to be 450.74 mg/L.Based on this value, it can be concluded that the test chemical 3,5 Diaminobenzoic acid may have no concern to micro organism toxicity for acute exposure period.

Last experimental study from authorative database (ECOTOX database) for read across 3-aminophenol (Cas no. 591-27-5) indicate the effect concentration (EC20) for the substance 3-Aminophenol (CAS no. 591-27-5) was determine to be 70 mg/l on micro organism species Tetrahymena thermophila (Ciliate Protozoa) on the basis of effect on Adenosine triphosphatase activity in static fresh water.

Thus all above available experimental and other supported reports arrive at a judgement as the test substance 3,5-Diaminobenzoic acid (CAS no. 535 -87 -5) may have showed toxic effect to micro organism for acute exposure time