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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from peer-reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Acute oral toxicity and repellency of 933 chemicals to House and Deer mice.
Author:
E.W.Schafer, Jr. and W.A.Bowles,jr
Year:
1985
Bibliographic source:
Arch. Environ.Contam.Toxicol. 14, 111-129 (1985)

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as below
Principles of method if other than guideline:
Acute oral toxicity and repellency study of 3,5-diaminobenzoic acid in mice
GLP compliance:
not specified
Test type:
other: No data
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3,5-diaminobenzoic acid
EC Number:
208-621-0
EC Name:
3,5-diaminobenzoic acid
Cas Number:
535-87-5
Molecular formula:
C7H8N2O2
IUPAC Name:
3,5-diaminobenzoic acid
Test material form:
solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3,5 Diaminobenzoic acid
- Molecular formula (if other than submission substance): C7H8N2O2
- Molecular weight (if other than submission substance): 152.1522 g/mole
- Substance type: Organic
- Physical state: Solid

Test animals

Species:
mouse
Strain:
other: Deer
Sex:
not specified
Details on test animals or test system and environmental conditions:
- Weight at study initiation: 20 gms
- Diet (e.g. ad libitum): wheat seed

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Water , corn oil, 1% carbopol
Details on oral exposure:
3,5 Diaminobenzoic acid was administrated by gavage using Water , corn oil, 1% carbopol as carriers.
Doses:
1250 mg/kg
No. of animals per sex per dose:
2 to 4 animals were used per geometrically spaced dosage.
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 3 days
Statistics:
estimated by method of thompson and weil

Results and discussion

Preliminary study:
No data
Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 1 250 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No mortality observed
Mortality:
No mortality was observed in treated mice at 1250 mg/kg
Clinical signs:
other: No data
Gross pathology:
No data
Other findings:
No data

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
LD50 was considered to be > 1250 mg/kg bw when Deer mice were treated with 3,5-diaminobenzoic acid orally by gavage.
Executive summary:

In a acute oral toxicity study, Deer mice were treated wtih 3,5-diaminobenzoic acid in the concentration of 1250 mg/kg bw orally by gavage using Water , corn oil, 1% carbopol as carriers and observed for 3 days. No 50 % mortality was observed in treated mice at 1250 mg/kg bw. Therefore,  LD50 was considered to be > 1250 mg/kg bw when Deer mice were treated with 3,5-diaminobenzoic acid orally by gavage.