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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to
Guideline:
other: Refer below principle
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the Petromyzon marinus (Sea Lamprey) in a 24 hr study period.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): butyl 12-acetoxyoctadec-9-enoate
- Molecular formula : C24H44O4
- Molecular weight : 396.6076 g/mol
- Smiles notation : CCCCCC[C@H](C\C=C/CCCCCCCC(=O)OCCCC)OC(=O)C
- InChl : 1S/C24H44O4/c1-4-6-8-15-18-23(28-22(3)25)19-16-13-11-9-10-12-14-17-20-24(26)27-21-7-5-2/h13,16,23H,4-12,14-15,17-21H2,1-3H3/b16-13-/t23-/m1/s1
- Substance type:Organic
- Physical state:Liquid
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Petromyzon marinus
Details on test organisms:
TEST ORGANISM
- Common name:Sea Lamprey
- Source:Fish Division of the Michigan Conservation Department
- Length at study initiation (length definition, mean, range and SD):3-5 inches or slightly less in length.
- Weight at study initiation (mean and range, SD):N/A
- Species Lifestage :Larva(e)




Test type:
static
Water media type:
freshwater
Total exposure duration:
24 h
Hardness:
Not reported
Test temperature:
55 Degree Fahrenheit
pH:
7.5-8.2
Dissolved oxygen:
8.6-13.7 parts per million
Salinity:
Not reported
Details on test conditions:
TEST SYSTEM
- Test vessel:Glass battery jar
- Material, size, headspace, fill volume: 5 liters
- Aeration: Provided with aeration through standard stone air - breakers
- No. of organisms per vessel:Usually six in number.
The aggregate test animals available, usually six in number, were placed together in a 10-liter glass battery jar containing 5 liters of water. These jars were provided with aeration through standard stone air - breakers and were maintained at a constant temperature by immersion in specially constructed constant temperature troughs. Water temperature was maintained within the limits of +/- 1. 0 degree Farenheit. Four such constant temperature units were utilized, each having a capacity of thirteen 10-liter battery Jars. Twelve of these test jars were included with one control jar in each trough. Fish in the control jar were exposed only to the water and physical conditions of the typical test container.The resultant volume in each test Jar varied from 5800 to 6200 cc.Test were conducted for a 4-hour period.

TEST MEDIUM / WATER PARAMETERS
- Free CO2 : 5.0 to 9.0 p.p.m.

OTHER TEST CONDITIONS
- Adjustment of pH : 7.5 to 8.2

Reference substance (positive control):
not specified
Key result
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: No adverse effect observed.
Conclusions:
In short term fish toxicity the no observed effect concentration (NOEC) value of butyl 12-acetoxyoctadec-9-enoate in fish[Petromyzon marinus] in a 24 hr study was obsereved to be 5 mg/L.
Executive summary:

This study was designed to assess the toxic effects of the test compound on the Petromyzon marinus (Sea Lamprey) in a 24 hr study period.

The test condition with a pH of 7.5 to 8.2 and test temperature 55 Degree Fahrenheit, Dissolved oxygen levels ranging from 8.6-13.7 parts per million was maintained. The aggregate test animals available, usually six in number, were placed together in a 10-liter glass battery jar containing 5 liters of water. These jars were provided with aeration through standard stone air - breakers and were maintained at a constant temperature by immersion in specially constructed constant temperature troughs. Water temperature was maintained within the limits of +/- 1. 0 degree Farenheit. Four such constant temperature units were utilized, each having a capacity of thirteen 10-liter battery jars. Twelve of these test jars were included with one control jar in each trough. Fish in the control jar were exposed only to the water and physical conditions of the typical test container. The resultant volume in each test Jar varied from 5800 to 6200 cc. Test were conducted for a 24-hour period.

In experiment, no any remarkable effect was noted; thus the no observed effect concentration (NOEC) value of butyl 12-acetoxyoctadec-9-enoate (Cas no.140 -04 -5) in fish[ Petromyzon marinus] in a 24 hr study was considered to be 5 mg/L.

Description of key information

This study was designed to assess the toxic effects of the test compound on the Petromyzon marinus (Sea Lamprey) in a 24 hr study period.

The test condition with a pH of 7.5 to 8.2 and test temperature 55 Degree Fahrenheit, Dissolved oxygen levels ranging from 8.6-13.7 parts per million was maintained. The aggregate test animals available, usually six in number, were placed together in a 10-liter glass battery jar containing 5 liters of water. These jars were provided with aeration through standard stone air - breakers and were maintained at a constant temperature by immersion in specially constructed constant temperature troughs. Water temperature was maintained within the limits of +/- 1. 0 degree Farenheit. Four such constant temperature units were utilized, each having a capacity of thirteen 10-liter battery jars. Twelve of these test jars were included with one control jar in each trough. Fish in the control jar were exposed only to the water and physical conditions of the typical test container. The resultant volume in each test Jar varied from 5800 to 6200 cc. Test were conducted for a 24-hour period.

In experiment, no any remarkable effect was noted; thus the no observed effect concentration (NOEC) value of butyl 12-acetoxyoctadec-9-enoate (Cas no.140 -04 -5) in fish[ Petromyzon marinus] in a 24 hr study was considered to be 5 mg/L.

Key value for chemical safety assessment

Additional information

Two studies including experimental results for short term fish toxicity endpoint of test substance butyl 12-acetoxyoctadec-9-enoate (CAS no.140-04-5) with relevant read across which is structurally similar to target were summarized as follows:

 

First experimental study (from Spec.Sci.Rep.Fish.No.207, Fish Wildl.Serv., U.S.D.I., Washington, DC:157 p.)for target chemical suggest study was designed to assess the toxic effects of the test compound on the Petromyzon marinus (Sea Lamprey) in a 24 hr study period.

The test condition with a pH of 7.5 to 8.2 and test temperature 55 Degree Fahrenheit, Dissolved oxygen levels ranging from 8.6-13.7 parts per million was maintained. The aggregate test animals available, usually six in number, were placed together in a 10-liter glass battery jar containing 5 liters of water. These jars were provided with aeration through standard stone air - breakers and were maintained at a constant temperature by immersion in specially constructed constant temperature troughs. Water temperature was maintained within the limits of +/- 1. 0 degree Farenheit. Four such constant temperature units were utilized, each having a capacity of thirteen 10-liter battery jars. Twelve of these test jars were included with one control jar in each trough. Fish in the control jar were exposed only to the water and physical conditions of the typical test container. The resultant volume in each test Jar varied from 5800 to 6200 cc. Test were conducted for a 24-hour period.

In experiment, no any remarkable effect was noted; thus the no observed effect concentration (NOEC) value of butyl 12-acetoxyoctadec-9-enoate (Cas no.140 -04 -5) in fish[ Petromyzon marinus] in a 24 hr study was considered to be 5 mg/L. Based on the value thebutyl 12-acetoxyoctadec-9-enoatewas considered to be non toxic to fish hence, it was considered to be not classified as per the CLP regulation.

Above aquatic study is supported by read across chemical Bis(2-ethylhexyl) adipate (Cas no.103-23-1). Short term toxicity to fish study was carried out for 96 hrs. according to OECD Guideline 203 (Fish, Acute Toxicity Test). for chemical Bis(2-ethylhexyl) adipate (Cas no.103-23-1). Based on effect on mortality of the fish, the 96 hr LC50 value was determined to be >50 mg/l. Based on the value the Bis(2-ethylhexyl) adipate was considered to be toxic to fish but as it was readily biodegradable in nature hence, Bis(2-ethylhexyl) adipate was considered to be not classified as per the CLP regulations. (J-CHECK database; 2017)

Thus all above available studies gives the final conclusion as target substancebutyl 12-acetoxyoctadec-9-enoate(Cas no.140-04-5) is expected to be non toxic to fish and thus not consider for the aquatic classification as per the CLP criteria.