Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs.

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2013-12-16 until 2014-01-28

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(1992)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all solutions with surviving fish
- Sample volume: 2.0 mL
- Sample storage conditions before analysis: stored in a freezer until analysis (≤ -15°C)

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: preparation of stock solutions (test substance concentrations: 0.46, 1.0, 2.2, 4.6, and 10.0 g/L)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (final test solution(s) including control(s)): 100 µg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, the Netherlands
- Age at study initiation: 4 months
- Length at study initiation (length definition, mean and SD): 2.5 ± 0.2 cm (final test), 3.7 ± 0.2 cm (range-finder)
- Weight at study initiation (mean and range, SD): 0.55 ± 0.15 cm (final test), 1.76 ± 0.17 cm (range-fin
- Method of breeding: F1 from a single parent-pair bred in UV-treated water
- Feeding during test: none

ACCLIMATION
- Acclimation period: at least 12 days after delivery
- Acclimation conditions (same as test or not): yes
- Type and amount of food: pelleted fish food (Cyprico crumble Excellent, 300-500 um, Coppens International bv. Helmond, NL)
- Feeding frequency: daily
- Health during acclimation (any mortality observed): < 5%

QUARANTINE (wild caught)
- not applicable

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

180 mg CaCO3/L

Test temperature:

20-24°C, constant within 2°C (continous measurement)

pH:

pH ISO-medium: 7.7 ± 0.3

Dissolved oxygen:

> 60% of air saturation value

Nominal and measured concentrations:

measured concentrations 69-82% of target during the test, except in the lowest concentration at test start (105%)

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L all-glass, containing 9.5 L of test solution
- Aeration: no aeration
- Renewal rate of test solution (flow rate): 6 L/h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.027 g fish per litre per day

TEST MEDIUM / WATER PARAMETERS
- Medium: Adjusted ISO medium
- Source/preparation of dilution water: GEON Waterbehandling, Berkel-Enschot, NL (tap-water purified by reverse osmosis)
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily in all vessels with surviving fish

OTHER TEST CONDITIONS
- Adjustment of pH: no, except if pH values of test solutions are outside the optimal pH range for the species to be tested.
- Photoperiod: 16 hours
- Light intensity: not specified

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: not applicable
- Range-finding test concentrations [mg/L] : 0.1, 1.0, 10, 100
- Test concentrations [mg/L]: 0.046, 0.10, 0.22, 0.46, 1.0

CONTROL
Test medium without test substance but with the additive used in the treatment of the stock solutions.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.17 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 0.13-0.24 mg/L

Details on results:

- Mortality of control: no
- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Reported statistics and error estimates:

Parameters of the probit analysis at 72 h and 96h
--------------------------------------------
Computation runs: 0.0000
Slope b: 6.7749
Intercept a: 5.1580
Variance of b: 0.9210
Goodness of Fit
Chi²: 5.8217
Degrees of freedom: 3.0000
p(Chi²): 0.1206
Log LC50: -0.76133
SE Log LC50: 0.0661
g-Criterion: 0.0771
F: 25.6820
p(F) (df: 1;3): 0.0150

Sublethal observations / clinical signs:

Results range-finding test

Nominal conc. [mg/L]	Initial no. of fish	Cumulative mortality					Total mortality [%]
		2 -4h	24h	48h	72h	96h
0.10	3	0	0	0	0	0	0
1.0	3	0	3	3	3	3	100
10	3	3	3	3	3	3	100
100	3	3	3	3	3	3	100

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 was 0.17 mg/L (95% confidence interval between 0.13 and 0.24 mg/L) and was already reached after 72 hours of exposure.

Executive summary:

96 -Hour Acute Toxicity Study in Carp with Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs..

The study procedure described is based on the OECD guideline No. 203, 1992. In addition, the procedures were designed to meet the test methods of the Comission Regulation (EC) No 440/2008, Part C.1, 2008 and the ISO International Standard 7346 -1: Static method, 1996.

The batch of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. tested was a dark brown viscous liquid with a purity of 89.2%. Stock solutions were prepared in acetone at concentrations a factor of 10,000 higher than the target concentrations.

A final test under flow-through conditions was performed based on the results of a preceding range-finding test under static conditions. The stock solutions were dosed via a computer-controlled system from the dilution water (ISO-medium) supply. The dilution water was supplied via a flow meter with a flow rate of 6 L/h. In the mixing flasks the dosed volumes and the dilution water were mixed under continuous stirring. Subsequently, the test solution coming from the mixing flask entered the vessel containing the fish. The whole system was checked daily.

Seven fish per concentration were exposed to a control and to target concentrations of 0.046, 0.10, 0.22, 0.46 and 1.0 mg/L. The total exposure period was 96 hours and samples for analytical confirmation of actual exposure concentrations were taken two days before the start of exposure, at the start, after 48 hours of exposure and at the end of the test (from the control and all concentrations with surviving fish). In addition samples were taken after 24 hours of exposure from the two highest test concentrations.

Analysis of the samples taken two days prior to the start of the test showed that the system was functioning adequately although most recoveries were somewhat low relative to the target concentrations, i.e. 62 -84% of target, with one exception for the lowest test concentration at the start of the test (105% of target). Given these results, the effect parameters were based on the average exposure concentrations that were calculated to be: 0.039, 0.075, 0.16, 0.34, and 0.81 mg/L.

The study met the acceptability criteria prescribed by the protocol and was considered valid.

The 96h-LC50 was 0.17 mg/L (95% confidence interval between 0.13 and 0.24 mg/L) and was already reached after 72 hours of exposure.

Description of key information

The short-term toxicity of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. was assessed in a 96h acute study in a continous system with carp.

The result of an 96h-LC50 of 0.17 mg/L (95% CL 0.13 -0.24 mg/L) already reached after 72 hours of exposure, can be considered reliable without restrictions.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.17 mg/L

Additional information

The obtained 96h-LC50 of 0.17 mg/L (95% CL 0.13 -0.24 mg/L) in fish is based on the average exposure concentrations. Acetone was used as solvent to enable stable concentration in the flow through system. This is not considered to have any impact on the result of the study, as the acute toxicity value (48h-EC50) for fresh water invertebrates lies in the same range.