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EC number: 944-528-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- A study on the microbial toxicity of the substance is not available. The ECHA guidance R.7b says that under certain conditions, the findings of the toxicity control in a study on the ready biodegradability can be used to derive a NOEC value for microbial activity. In the available, reliable study on ready biodegradability, no inhibition of microbiologic activity was seen in the toxicity control. The results of this toxicity are used to define the NOEC value and this approach is considered as reliable with restrictions.
- Reason / purpose for cross-reference:
- reference to other study
- Principles of method if other than guideline:
- The toxicity of the substance to microbial activity was tested in the context of a ready biodegradability study in accordance with OECD 301D. The substance and a positive reference substance were added to a testing flask together with inoculum from a municipal sewage treatment plant. The biodegradation of the positive reference substance over the period of the degradability study was monitored.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Hildesheim, Germany
- Laboratory culture: no
- Pretreatment: Activated sludge was filtered with folded filter. The first 200 mL of the filtrate were not used. The second filtrate effluent from the domestic waste water is used to initiate inoculation.
- Initial cell/biomass concentration: 10E+4 - 10E+6 Colony Forming Units (CFU)/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 19-21 °C
- pH:
- 7.2 -7.6
- Dissolved oxygen:
- The dissolved oxygen concentration was greater 6 mg/L over the period of 14 days
- Nominal and measured concentrations:
- 1.5 mg/L test item
5.0 mg/L sodium acetate (positive reference item) - Details on test conditions:
- Ten bottles containing the test substance, positive reference substance and inoculum were tested (Toxicity control)
- Reference substance (positive control):
- no
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- A degradation of 31% was observed after 14 days, which is above the threshold value of 25% given in the OECD TG.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is not inhibitory to the microbial activity of activated sludge at a nominal concentration of 1.5 mg/L.
- Executive summary:
The microbial activity of the test substance was studied in the context of a ready biodegradability test according to OECD 301D by conducting a toxicity control. Test vessels containing 1.5 mg/L test substance, 5.0 mg/L sodium acetate (positive reference control) and the inoculum (acitivated sludge from a municipal sewage treatment plant processing domestic sewage) were incubated under aerobic conditions in the dark at 20 °C for a period of 28 days. The degradation of the test and reference substance was monitored by determining the dissolved oxygen concentration regularly. A degradation of 31% was observed after a period of 14 days. The threshold limit value of 25% after 14 days hence was exceeded and the substance did not significantly inhibit the microbial activity. A NOEC value of 1.5 mg/L for microbial activity can be derived from this study.
Reference
Description of key information
Experimental data on the toxicity of the test substance to microorganisms are not available. The toxicity control in the ready biodegradability study (OECD 301D) did not inhibit the activity of the inoculum at a concentration of 1.5 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1.5 mg/L
Additional information
The microbial activity of the test substance was studied in the context of a ready biodegradability test according to OECD 301D by conducting a toxicity control. Test vessels containing 1.5 mg/L test substance, 5.0 mg/L sodium acetate (positive reference control) and the inoculum (acitivated sludge from a municipal sewage treatment plant processing domestic sewage) were incubated under aerobic conditions in the dark at 20 °C for a period of 28 days. The degradation of the test and reference substance was monitored by determining the dissolved oxygen concentration regularly. A degradation of 31% was observed after a period of 14 days. The threshold limit value of 25% after 14 days hence was exceeded and the substance did not significantly inhibit the microbial activity. A NOEC value of 1.5 mg/L for microbial activity can be derived from this study.
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