Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 942-871-8 | CAS number: 1799569-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Juny 15, 2016 - September 2, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-ethoxy-4,6-difluoro-7-(pentyloxy)dibenzo[b,d]furan
- EC Number:
- 942-871-8
- Cas Number:
- 1799569-89-3
- Molecular formula:
- C19H20F2O3
- IUPAC Name:
- 3-ethoxy-4,6-difluoro-7-(pentyloxy)dibenzo[b,d]furan
1
- Specific details on test material used for the study:
- Designation: Art. 201386
Synonym: B-2O-O5
Batch: EF13004186
Purity (HPLC): 100.0%
Appearance: White, solid
Water solubility: <0.00003 g/L (OECD 105)
Stability under test conditions: Not specified
Date of expiry: April 30, 2018
Storage: Tightly closed, dark at room temperature (15 to 25°C)
Preparation of the Test Item:
A stock preparation with a test item concentration of 100 mg/L was freshly prepared. For that purpose, the test item was weighed in a calibrated flask and vehicle was added. The preparation was treated in an ultrasonic device for 30 minutes. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the formulation was passed through a membrane filter with a pore size of 0.2 µm. The filtrate was used for the study.
Sampling and analysis
- Analytical monitoring:
- no
- Remarks:
- The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (<0.00003 g/L), the compound cannot be detected with standard analytical methods.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Macro nutrients (mg/L)
CaCI2 •2H20 293.80
MgSO4•7H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3•9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Trace elements (mg/L)
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients (mg/L)
Na2EDTA • 2H20 2.50
Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75
- pH: 7.89
- Total hardness: 220 mg CaCO3/L
Preparation of the Test Item:
A stock preparation with a test item concentration of 100 mg/L was freshly prepared. For that purpose, the test item was weighed in a calibrated flask and vehicle was added. The preparation was treated in an ultrasonic device for 30 minutes. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the formulation was passed through a membrane filter with a pore size of 0.2 µm. The filtrate was used for the study.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna Straus
Origin: Daphnia magna Straus was originally obtained from IBACON GmbH (Roßdorf, Germany).
Culture conditions: The clone is bred in the laboratories of Merck KGaA (room 2068).
Parental daphnids are used for reproduction until they are about 6 weeks old. Thereafter, they are replaced by neonates.
Daphnids are kept individually in 100 mL glass vessels containing approximately 60 mL reconstituted water (ELENDT M4 medium) at a water temperature of 20 ± 2°C and a 16 hour light and 8 hour dark regime to ensure similar conditions as in the experiment. Offspring are removed from the vessels at least twice per week.
Feeding: The parental daphnids are fed ad libitum with unicellular green algae Desmodesmus subspicatus three times per week.
Age: Offspring less than 24 hours old were used for the study.
Acclimation period: same as test
Feeding during test: None
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 220 mg CaCO3/L
- Test temperature:
- 19.2 – 19.9°C
- pH:
- 7.89 - 7.91
- Dissolved oxygen:
- 7.72 - 8.35
- Conductivity:
- 802 µS/cm
- Nominal and measured concentrations:
- Nominal 100 mg/L
- Details on test conditions:
- EXPOSURE:
The study was performed in an air-conditioned room. For the study 20 mL glass test tubes containing at least 20 mL either reconstituted water (control group) or test medium (test item group) were used. Each test vessel contained five daphnids resulting in 4 mL medium per daphnid. They were not fed and the media were not aerated during the exposure.
The test was performed as a static test in open vessels.
The duration of exposure was 48 hours. During the exposure period, the mobility of the daphnids was assessed daily, i.e. after 24 and 48 hours.
NO. OF DAPHNIDS:
Control Group: 20 daphnids
100 mg/L: 20 daphnids
CONCENTRATION(S)
In a pre-test no immobilization was observed at a concentration of 100 mg/L under open static conditions. Therefore, the solution of a nominal test item concentration of 100 mg/L was tested in the present study.
For the control, reconstituted water (ELENDT M4 medium) was used.
VEHICLE
Reconstituted water (ELENDT M4 medium) was used as vehicle.
Macro nutrients (mg/L)
CaCI2 •2H20 293.80
MgSO4•7H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3•9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Trace elements (mg/L)
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients (mg/L)
Na2EDTA • 2H20 2.50
Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75
- pH: 7.89
- Total hardness: 220 mg CaCO3/L
- O2-Concentration: 8.35 mg/L
- Conductivity: 802 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: 48 h
OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark
- Light intensity: The mean light intensities were 614 Lux prior to and at the end of the exposure period, respectively.
References:
ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990 - Reference substance (positive control):
- no
- Remarks:
- No positive control used in this study. The accuracy and reliability of the test method is demonstrated periodically as recommended by guidelines with potassium dichromate.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: EC50 > 3.0E-5 g/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: EC50 > 3.0E-5 g/L
- Details on results:
- Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of Art. 201386 (B-2O-O5) revealed no aquatic toxicity in the test system.
The 48h EC50 exceeded the water solubility of 0.00003 g/L (nominal >100 mg/L) and, thus, could not be determined in this study.
- Behavioural abnormalities: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no - Results with reference substance (positive control):
- Potassium dichromate:
24h EC50 1.00 (0.85 - 1.23) mg/L
48h EC50 0.66 (could not be determined) mg/L
The test item Art. 104864 (Potassium dichromate) showed a 24h EC50 value of 1.0 mg/L which is within the range of the published data of 0.6 to 1.7 mg/L (Council Regulation (EC) No. 440/2008) and 0.6 to 2.1 mg/L (OECD Guideline No. 202).
Any other information on results incl. tables
Objective
The objective of this study was to determine the effect of the test item Art. 201386 (B-2O-O5) on the immobilization of Daphnia magna.
Study Design
Juvenile daphnids were exposed to a nominal test item concentration of 100 mg/L (limit test) in an open static system. 20 daphnids, divided into four replicates, each with five animals were used in the test item group and the control group. The daphnids were observed for immobilization after 24 and 48 hours of exposure.
Results
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (<0.00003 g/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is complex.
Due to absence of any adverse effects at the saturation concentration, the study was performed without analytical verification.
No effect on the mobility of the daphnia was observed at a nominal concentration of 100 mg/L.
For the test item Art. 201386 (B-2O-O5) the following EC50 values were determined:
EC50 (24h) >0.00003 g/L (nominal >100 mg/L)
EC50 (48h) >0.00003 g/L (nominal >100 mg/L)
Conclusion
Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of Art. 201386 (B-2O-O5) revealed no aquatic toxicity in the test system.
The
48h EC50was >0.00003 g/L (nominal >100 mg/L) and, thus, could
not be determined in this study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of Art. 201386 (B-2O-O5) revealed no aquatic toxicity in the test system.
The 48h EC50 was >0.00003 g/L (nominal >100 mg/L) and, thus, could not be determined in this study. - Executive summary:
This study was performed according to GLP and the methods applied are fully compliant with OECD TG 202. Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of Art. 201386 (B-2O-O5) revealed no aquatic toxicity in the test system. The 48h EC50 exceeded the water solubility of 0.00003 g/L (nominal >100 mg/L) and, thus, could not be determined in this study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.