2-ethylhex-2-enal

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restriction (e.g. non-GLP, no analytical verification of test item concentrations)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38 412 L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhex-2-enal

EC Number:

211-448-3

EC Name:

2-ethylhex-2-enal

Cas Number:

645-62-5

Molecular formula:

C8H14O

IUPAC Name:

2-ethylhex-2-enal

Details on test material:

- Name of test material (as cited in study report): 2-Ethylhexen-2-al crude
- Analytical purity: >= 95 %)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria. No undissolved test substance was visible.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range: 6.0-7.2 cm)
- Weight at study initiation (mean and range, SD): 2.8 g (range: 2.0-3.6 g)
- Feeding during test: 1 day before and during exposure

ACCLIMATION
- Acclimation period: 3 days

Medical treatment during housing: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

20 °C

pH:

7.4 - 7.8

Dissolved oxygen:

8.1 - 8.8 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 0 (control), 4.64, 10.0, 21.5, 46.4, 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: non-sealed all-glass aquaria (30x22x24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.8 g fish / l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater was prepared from fully demineralized tap water according to DIN 38 412, part 11 (Oct 1982) that was resalted by the addition of 294.0 mg/l CaCl2*2H2O, 123.3 mg/l MgSO4*7H2O, 63.0 mg/l NaHCO3 and 5.5 mg/l KCl.
- Acid capacity: 0.8 mmol/l
- Na/K ions: 10:1
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: measurements: temperature, pH, and oxygen content after 1, 24, 48, 72, and 96 h


OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms after 1, 4, 24, 48, 72, and 96 h

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: symptoms of intoxication

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

LC50 (48 h): 32 mg/l (this lethal concentration corresponds to the normal sensitivity)

Reported statistics and error estimates:

Probit analysis (Finney 1971). The LC50 value was calculated as the geometrical mean of the 10.0 mg/l and the 21.5 mg/l value.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality:
Nominal test Cumulative mortality (n) after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) 0 0 0 0 0 0
4.64 0 0 0 0 0 0
10.0 0 1 1 1 1 1
21.5 0 10 10 10 10 10
46.4 0 10 10 10 10 10
100.0 0 10 10 10 10 10


Symptoms of intoxication:
Nominal test Symptoms of intoxication after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) - - - - - -
4.64 - - - - - -
10.0 - - - - - -
21.5 NT + + + + +
46.4 + + + + + +
100.0 + + + + + +

Definitions of Symptoms:
-: No symptoms
+: All fish dead
N: Narcotic-like state
T: Tumbling

pH:
Nominal test pH after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) 7.5 7.7 7.7 7.8 7.7
4.64 7.4 7.5 7.6 7.5 7.6
10.0 7.4 7.4 7.5 7.5 7.5
21.5 7.4
46.4 7.4
100.0 7.4

Dissolved oxygen:
Nominal test dissolved oxygen (mg/l) after x hours
concentration 1 4 24 48 72 96
(mg/l)
0 (control) 8.1 8.3 8.6 8.1 8.2
4.64 8.1 8.5 8.8 8.4 8.2
10.0 8.3 8.8 8.5 8.4 8.2
21.5 8.5
46.4 8.6
100.0 8.7

Temperature:
The temperature remained constantly at 20 °C in all test vessels throughout the test.