2-ethylhex-2-enal

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study, with acceptable restrictions (e.g. non-GLP, no analytical verification of test item concentrations).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German Standard DIN 38412, Part 8 (draft)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhex-2-enal

EC Number:

211-448-3

EC Name:

2-ethylhex-2-enal

Cas Number:

645-62-5

Molecular formula:

C8H14O

IUPAC Name:

2-ethylhex-2-enal

Details on test material:

- Name of test material (as cited in study report): 2-Ethylhexen-2-al crude

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution of test item: the test item was dispersed with the addition of Tween 80 (125 mg/l) by stirring (2 h at 20 °C)
- Controls: test medium with 125 mg/l Tween 80

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

Pseudomonas putida, DSM 50026. Inoculum: 1 ml pre-culture (adjusted to standardized turbidity)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Test temperature:

incubated at 20 °C (293 K)

pH:

5.6 - 7.1 (inoculated)
5.0 - 6.1 (uninoculated)

Nominal and measured concentrations:

Nominal test concentrations (mg/l): 0 (control + solvent control), 7.81, 15.63, 31.25, 62.5, 125, 250, 500, 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 ml-Erlenmeyer flasks, 1 baffle
- Fill volume: 100 ml
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK-medium according to DIN 38412, Part 8 (draft); 10 ml (0.5 ml nutrient solution concentrate I (20x), 0.5 ml nutrient solution concentrate II (20x), 1 ml inoculum, 8 ml test item solution)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
photometric determination at 436 nm after 17 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

17 h

Dose descriptor:

other: EC90

Effect conc.:

490 mg/L

Effect concentrations 2

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

320 mg/L

Effect concentrations 3

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

405 mg/L

Any other information on results incl. tables

Concentration (mg/l)   Growth (% of control)
   0 (control)         100
   0 (solvent   
      control)         100
   7.81                106.3
  15.63                111.72  
  31.25                115.77
  62.5                 117.58
 125                   126.56
 250                   123.13
 500                     5.05
1000                     0.5


pH at test start and test end:

test conc.      uninoculated   uninoculated
(mg/l)          (0 h)          (17 h)
   0 (control)  7.1            5.3
   0 (solvent   
      control)  7.1            5.2
   7.81         7.1            5.2
  15.63         7.1            5.1
  31.25         7.1            5.3
  62.5          7.0            5.2
 125            6.9            5.0
 250            6.8            5.0
 500            6.5            6.1
1000            5.6            5.7