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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, with acceptable restrictions (e.g. non-GLP, no analytical verification of test item concentrations).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German Standard DIN 38412, Part 8 (draft)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethylhex-2-enal
EC Number:
211-448-3
EC Name:
2-ethylhex-2-enal
Cas Number:
645-62-5
Molecular formula:
C8H14O
IUPAC Name:
2-ethylhex-2-enal
Details on test material:
- Name of test material (as cited in study report): 2-Ethylhexen-2-al crude

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution of test item: the test item was dispersed with the addition of Tween 80 (125 mg/l) by stirring (2 h at 20 °C)
- Controls: test medium with 125 mg/l Tween 80

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida, DSM 50026. Inoculum: 1 ml pre-culture (adjusted to standardized turbidity)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Test temperature:
incubated at 20 °C (293 K)
pH:
5.6 - 7.1 (inoculated)
5.0 - 6.1 (uninoculated)
Nominal and measured concentrations:
Nominal test concentrations (mg/l): 0 (control + solvent control), 7.81, 15.63, 31.25, 62.5, 125, 250, 500, 1000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 ml-Erlenmeyer flasks, 1 baffle
- Fill volume: 100 ml
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK-medium according to DIN 38412, Part 8 (draft); 10 ml (0.5 ml nutrient solution concentrate I (20x), 0.5 ml nutrient solution concentrate II (20x), 1 ml inoculum, 8 ml test item solution)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
photometric determination at 436 nm after 17 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
490 mg/L
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
320 mg/L
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
405 mg/L

Any other information on results incl. tables

Concentration (mg/l)   Growth (% of control)
0 (control) 100
0 (solvent
control) 100
7.81 106.3
15.63 111.72
31.25 115.77
62.5 117.58
125 126.56
250 123.13
500 5.05
1000 0.5


pH at test start and test end:

test conc. uninoculated uninoculated
(mg/l) (0 h) (17 h)
0 (control) 7.1 5.3
0 (solvent
control) 7.1 5.2
7.81 7.1 5.2
15.63 7.1 5.1
31.25 7.1 5.3
62.5 7.0 5.2
125 6.9 5.0
250 6.8 5.0
500 6.5 6.1
1000 5.6 5.7

Applicant's summary and conclusion

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