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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Code of Federal Regulations, Title 16, Section 1500.42
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrazole
EC Number:
206-017-1
EC Name:
Pyrazole
Cas Number:
288-13-1
Molecular formula:
C3H4N2
IUPAC Name:
1H-pyrazole
Details on test material:
no further data

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
Breeder: W. Ostrop / Meerbusch
The animals were caged single and had "mümmel z" (ssniff / Soest) diet and water ad libitum.
Temperature 16 - 18 °C, Humidity 40 %
12 h Light/12 h Dark-Cycle
Acclimatization period 7 - 14 d

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
100 µl
Duration of treatment / exposure:
single treatment
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
3
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): not rinsed


SCORING SYSTEM: OECD/Draize score

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24-72 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Remarks:
within 8 days
Remarks on result:
other: see table for details
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24-72 h
Score:
1
Max. score:
2
Reversibility:
not reversible
Remarks:
within 8 days
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24-72 h
Score:
3
Max. score:
3
Reversibility:
not fully reversible within: 8 days
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24-72 h
Score:
4
Max. score:
4
Reversibility:
not fully reversible within: 8 days

Any other information on results incl. tables

Pyrazol caused a light corneal opacity, iritis, strong erythema and edema of the conjunctivae and increased lacrimation. The clear irritation effects at the eye were only partly reversible within 8 days.

Readings

Animal

Cornea

Iris

Conjunctiva

Additional findings

op

ar

red

ch

di

24 h

1

1

4

1

3

4

3

2

1

4

1

3

4

3

3

1

4

1

3

4

3

48 h

1

1

4

1

3

4

3

2

1

4

1

3

4

3

3

1

4

1

3

4

3

72 h

1

1

4

1

3

4

3

2

1

4

1

3

4

3

3

1

4

1

3

4

3

8 d

1

1

2

1

2

2

3

2

1

2

1

3

2

3

3

1

4

1

3

4

3

Mean

24 -72 h

1

1

4

1

3

4

3

2

1

4

1

3

4

3

3

1

4

1

3

4

3

Mean

24-72 h

1-3

1

4

1

3

4

3

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Remarks:
Migrated information
Conclusions:
Regarding the results of the study, the test substance pyrazole causes irreversible damage to the eyes.