Tetrylammonium bromide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the ZEBRA FISH (Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation :average 1.63 cm
- Weight at study initiation (mean and range, SD):0.1899g (average)
ACCLIMATION- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):During last 48 hours = 0FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:Withdrawal of food 1 day before and during exposure

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.05

Dissolved oxygen:

8.0 mg/l

Nominal and measured concentrations:

control and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: The test vessels used were all plastic aquaria having the water capacity of 5liters.
fill volume: 2 liters
- No. of organisms per vessel:8 fishes
OTHER TEST CONDITIONS - Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

 

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
			24 h		48h		72h		96h
Control	4	4	8.16	8.17	8.01	8.07	7.95	7.99	7.66	7.74
100	4	4	8.36	8.37	8.12	8.13	8.27	8.30	7.52	7.57

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24 h		48h		72h		96h
Control	4	4	7.01	7.01	6.99	6.99	5.99	5.99	6.21	6.27
100	4	4	6.96	6.96	6.86	6.86	5.83	5.89	5.83	5.87

Test temperature

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
			24 h		48h		72h		96h
Control	4	4	23.1	23.2	23.9	23.9	23.9	23.9	23.9	23.8
100	4	4	23.4	23.4	23.7	23.7	23.5	23.4	23.2	23.1

 

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on ZEBRA FISH(Danio rerio)was determine to be >100 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Based on the data obtain from various sources for the target chemical and for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of target chemical on the mortality of fishes. The studies are as mentioned below:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

First study was supported by the second from experimental report. This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio). 5 liters Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The experimental phase of the main study started at 06-11-2017 and lasted for a period of 96 hrs. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L, 25mg/L, 50mg/L,100mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After completion of stirring test solution prepare for achieving test concentrations. No effect were observed at 100 mg/l nominal concentration. After 96 hours of exposure of test itemTetraethylammonium Chloride on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra Fish Danio rerio was determine to be > 100 mg/l. Based on the LC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Thus based on the overall studies it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.