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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the ZEBRA FISH(Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The experimental phase of the main study started at 27-02-2017 and lasted for a period of 96 hrs.
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): N,N,N-tributylbutan-1-aminium iodide
- Molecular formula (if other than submission substance): C16H36N.I
- Molecular weight (if other than submission substance): 369.367 g/mol
- Smiles notation (if other than submission substance): [N+](CCCC)(CCCC)(CCCC)CCCC.[IH-]
- InChI: 1S/C16H36N.HI/c1-5-9-13-17(14-10-6-2,15-11-7-3)16-12-8-4;/h5-16H2,1-4H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 1 hr continuous stirring for achieving test concentrations of 100mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation (length definition, mean, range and SD):average 1.63 cm
- Weight at study initiation (mean and range, SD):0.1899g (average)

ACCLIMATION
- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):During last 48 hours = 0

FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:Withdrawal of food 1 day before and during exposure
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
148.5 mg of CaCO3
Test temperature:
23.5 °C
pH:
7.15
Dissolved oxygen:
7.9 mg/l
Nominal and measured concentrations:
control and 100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- No. of organisms per vessel:8 fishes

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark


Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

 

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.311-28-4 on ZEBRA FISH(Danio rerio)was found to be > 100 mg/L.
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Tetrabutylammonium iodide The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours.

The lethal concentrations LC50 was found to be >100 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = No mortality was observed

LC50 (96 hours) Experimental = >100 mg/L

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Tetrabutylammonium iodide The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours.

The lethal concentrations LC50 was found to be >100 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = No mortality was observed

LC50 (96 hours) Experimental = >100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Two studies including experimental and predicted data for short term fish toxicity of target chemical 1-Butanaminium, N,N,N-tributyl-, iodide (1:1) (Cas no.311-28-4) were summarised as follows:

Key study from UERL laboratory result;2017 suggest the Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Tetrabutylammonium iodide The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L and LC0 (96 hours) (highest loading at which no mortality was observed) = No mortality was observed whereas LC50 (96 hours) Experimental = >100 mg/L.

And supporting report from EPI Suite ECOSAR version 1.10 predicted model, the 96 hours LC50 was estimated to be 551.025 mg/l on Fish for substance N,N,N-tributylbutan-1-aminium iodide (CAS no.311-28-4) on the basis of mortality effects using neutral organics class for prediction.Based on this value, it can be concluded that the test chemical N, N, N-tributylbutan-1-aminium iodide (CAS no 311-28-4) can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered to be not classified as per the CLP regulations.

Thus based on the both results for short term fish concluded that the test substance 1-Butanaminium, N,N,N-tributyl-, iodide (1:1) (Cas no.311-28-4) was non toxic to fish for acute exposure thus not consider for the aquatic classification as per the CLP criteria.