Tetrabutylammonium iodide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data from peer reviewed journal.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Determination of short term toxicity to aquatic invertebrates using OECD guideline 202.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): N, N, N-tributylbutan-1-aminium iodide
- Molecular formula: C16 H36 N.I
- Molecular weight: 369.367 g/mol
- Smiles notation: [N+] (CCCC)(CCCC)(CCCC)CCCC.[IH-]
- Inchl : 1S/C16H36N.HI/c1-5-9-13-17(14-10-6-2,15-11-7-3)16-12-8-4;/h5-16H2,1-4H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Solid

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: born from parthenogenic females grown in a batch culture
- Age at study initiation (mean and range, SD): <24 hrs

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

20±1 ᵒC

Details on test conditions:

TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: 100 ml containing 40 ml of test solution.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): two replicate beakers for each of eight treatment concentrations

OTHER TEST CONDITIONS
- Light intensity: 16/8 h light/dark photoperiod

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : for immobilized individuals at 24 and 48 h after the beginning of the test

TEST CONCENTRATIONS
- Range finding study:
Three consecutive trials were carried out for each substance, for a total of six replicates at each concentration.
- Test concentrations: eight treatment concentrations (control plus seven toxicant concentrations)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

After the experiment, the EC 50 value for N, N, N-tributylbutan-1-aminium iodide (311-28-4) was determined to be 2.8 mg/l for 48 hrs. Based on value , N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category.

Executive summary:

Short term toxicity to aquatic invertebrates was performed according to the OECD guideline 202. The test was performed in 5 neonates having age of <24 hr in static fresh water condition for 20 degree C under 6 different concentrations. 100 ml beaker with 40 ml of test solution was taken for experiment. The temperature was 20 1 1C, with a 16/8 h light/dark photoperiod. Each test vessel was checked for immobilized individuals at 24 and 48 h after the beginning of the test.After the experiment, the EC 50 value for N, N, N-tributylbutan-1-aminium iodide (311-28-4) was determined to be 2.8 mg/l for 48 hrs. Based on value , N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category.

Description of key information

Short term toxicity to aquatic invertebrates was performed according to the OECD guideline 202. The test was performed in 5 neonates having age of <24 hr in static fresh water condition for 20 degree C under 6 different concentrations. 100 ml beaker with 40 ml of test solution was taken for experiment. The temperature was 20 1 1C, with a 16/8 h light/dark photoperiod. Each test vessel was checked for immobilized individuals at 24 and 48 h after the beginning of the test.After the experiment, the EC 50 value for N, N, N-tributylbutan-1-aminium iodide (311-28-4) was determined to be 2.8 mg/l for 48 hrs. Based on value , N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.8 mg/L

Additional information

Short term toxicity to aquatic invertebrates was summaries with one experimental data and one prediction report for target is as follows:

 

Study was performed on N,N,N-tributylbutan-1-aminium iodide by Paola Galletti et,al, New Journal of Chemistry,2009, 33, 1859–1868, 11th February 2009, according to the OECD guideline 202. The test was performed in 5 neonates having age of <24 hr in static fresh water condition for 20 degree C under 6 different concentrations. 100 ml beaker with 40 ml of test solution was taken for experiment. The temperature was 20 1 1C, with a 16/8 h light/dark photoperiod. Each test vessel was checked for immobilized individuals at 24 and 48 h after the beginning of the test. After the experiment, the EC 50 value for N, N, N-tributylbutan-1-aminium iodide (311-28-4) was determined to be 2.8 mg/l for 48 hrs. Based on value, N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category as per the CLP regulation.

 

Prediction was done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for N, N, N-tributylbutan-1-aminium iodide (CAS:311-28-4). EC50 value was estimated to be 56.57 mg/L on the basis of intoxication for Daphnia magna for 48 hrs duration. Based on value, N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 3 category as per the CLP regulation.

 

Based on the prediction data for N,N,N-tributylbutan-1-aminium iodide, it was classified in aquatic chronic 3 category but as experimental data suggested aquatic chronic 2 category classification for N,N,N-tributylbutan-1-aminium iodide, so it can be concluded that N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category as per the CLP regulation.