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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): N,N,N-tributylbutan-1-aminium iodide
- Molecular formula (if other than submission substance): C16H36N.I
- Molecular weight (if other than submission substance): 369.367 g/mol
- Smiles notation (if other than submission substance): [N+](CCCC)(CCCC)(CCCC)CCCC.[IH-]
- InChI: 1S/C16H36N.HI/c1-5-9-13-17(14-10-6-2,15-11-7-3)16-12-8-4;/h5-16H2,1-4H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Vibrio fisheri
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
15 min
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates):triplicates
Reference substance (positive control):
not specified
Key result
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
284 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
Results of the Student–Newman–Keuls post-hoc statistical test for pairwise comparison of EC50 (mmol L/1).
Reported statistics and error estimates:
The differences tested by ANOVA are highly significant (P o 0.001), moreover, the post-hoc test.
Validity criteria fulfilled:
not specified
Conclusions:
Vibrio fischeri in a 15 min inhibition of bioluminescence test the 50% effect concentrations (EC50) of the ammonium salts TBAI was observed to be 284 mg/l with 95% confidence limit (241–336)
Executive summary:

Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual.

The EC50 values of the tested ammonium salts for V. fischeri range from 1.3 to 284 mg L1. The differences tested by ANOVA are highly significant (P o 0.001).

In experiment the 50% effect concentrations (EC50) of the ammonium salts TBAI to Vibrio fischeri in a 15 min inhibition of bioluminescence test was observed to be 284 mg/l with 95% confidence limit (241–336).

Description of key information

Vibrio fischeri in a 15 min inhibition of bioluminescence test the 50% effect concentrations (EC50) of the ammonium salts TBAI was observed to be 284 mg/l with 95% confidence limit (241–336)

Key value for chemical safety assessment

EC50 for microorganisms:
284 mg/L

Additional information

Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual.

The EC50 values of the tested ammonium salts for V. fischeri range from 1.3 to 284 mg L1. The differences tested by ANOVA are highly significant (P o 0.001).

In experiment the 50% effect concentrations (EC50) of the ammonium salts TBAI to Vibrio fischeri in a 15 min inhibition of bioluminescence test was observed to be 284 mg/l with 95% confidence limit (241–336).