2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
1. To evaluate the acute toxicity of test material on Fathead Minnow fish.
2.To evlaute short term toxicity to fish for the test material

GLP compliance:

not specified

Specific details on test material used for the study:

IUPAC: 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one
Molecular Formula: C16H8O2S2
Molecular Weight: 296.369 g/mol
Smiles:O=C1c2c(S\C1=C1/Sc3c(cccc3)C1=O)cccc2
InChI:1S/C16H8O2S2/c17-13-9-5-1-3-7-11(9)19-15(13)16-14(18)10-6-2-4-8-12(10)20-16/h1-8H/b16-15-

Details on sampling:

1)composite stored a t 20 C i n dark.
Stock solution refrigerated

Test organisms (species):

other: 1) Pimephales promelas 2) Oryzias latipes

Details on test organisms:

1) TEST ORGANISM
- Common name: Fathead Minnow
- Source: Berry water gardens , windmill fish hatcheries , North Carolina
- Length at study initiation : 5.2 cm
- Weight at study initiation: 1.3 g
- Feeding during test: starved 48 hrs before test

ACCLIMATION
- Acclimation period: 10 days
- Acclimation conditions : maintained in plastic stock tank in continuously running charcoal –sand filtered Chapel Hill tap water
- Type and amount of food: protein enriched fish food
- Feeding frequency: 5-7 times per week

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

1) Observations were made after every 24 hs

Hardness:

1) 32mg/l

Test temperature:

1) 17°C

pH:

1) 6.8-8.5

Nominal and measured concentrations:

1) 0 , 18 , 32 ,56 , 100 , 180 mg/l

Details on test conditions:

1) Test vessel: 5-gallon wide mouth glass jar
- No. of organisms per vessel: 10

Reference substance (positive control):

not required

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Conclusions:

The test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one is not likely to be toxic to fish atleast in the concentration range of 180-1000 mg/l.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one (522 -75 -8).The studies are as mentioned below:

1. Test material was subjected to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline . The nominal concentration were0 , 18 , 32 ,56 , 100 , 180 mg/l

 Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l.

2. Short term toxicity test on fish was performed to evaluate potential of toxicity of test material on Oryzias latipes.The median lethel effect concentration (LC50) was noted at 24 , 72 , 96 h . The LC50 for test material was observed to be >1000 mg/l after 96 h. Based on this value it can be conluded that test material is not toxic to fish and can not be classified as per CLP criteria.

By considering LC50 value it is concluded that test chemical is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria.

Thus, based on the above summarised studies, 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is greater than 1000 mg/L. Thus, comparing this value with the criteria of CLP regulation, 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one cannot be classified

for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similar

read across, test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one is not likely to be toxic atleast in the range concentration range of 1000mg/L .

Description of key information

Short term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one (522 -75 -8).The studies are as mentioned below:

1. Test material was subjected to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline . The nominal concentration were0 , 18 , 32 ,56 , 100 , 180 mg/l

 Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l.

2. Short term toxicity test on fish was performed to evaluate potential of toxicity of test material on Oryzias latipes.The median lethel effect concentration (LC50) was noted at 24 , 72 , 96 h . The LC50 for test material was observed to be >1000 mg/l after 96 h. Based on this value it can be conluded that test material is not toxic to fish and can not be classified as per CLP criteria.

By considering LC50 value it is concluded that test chemical is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria.

Thus, based on the above summarised studies, 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is greater than 1000 mg/L. Thus, comparing this value with the criteria of CLP regulation, 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one cannot be classified

for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similar

read across, test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one is not likely to be toxic atleast in the range concentration range of 1000mg/L .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

Short term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one (522 -75 -8).The studies are as mentioned below:

1. Test material was subjected to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline . The nominal concentration were0 , 18 , 32 ,56 , 100 , 180 mg/l

 Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l.

2. Short term toxicity test on fish was performed to evaluate potential of toxicity of test material on Oryzias latipes.The median lethel effect concentration (LC50) was noted at 24 , 72 , 96 h . The LC50 for test material was observed to be >1000 mg/l after 96 h. Based on this value it can be conluded that test material is not toxic to fish and can not be classified as per CLP criteria.

By considering LC50 value it is concluded that test chemical is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria.

Thus, based on the above summarised studies, 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is greater than 1000 mg/L. Thus, comparing this value with the criteria of CLP regulation, 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one cannot be classified

for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similar

read across, test chemical 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one is not likely to be toxic atleast in the range concentration range of 1000mg/L .