[1aR-(1aα,4aα,7α,8aS*)]-octahydro-4,4,8,8-tetramethyl-4a,7-methano-4aH-naphth[1,8a-b]oxirene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 2016-March 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[1aR-(1aα,4aα,7α,8aS*)]-octahydro-4,4,8,8-tetramethyl-4a,7-methano-4aH-naphth[1,8a-b]oxirene

EC Number:

247-851-6

EC Name:

[1aR-(1aα,4aα,7α,8aS*)]-octahydro-4,4,8,8-tetramethyl-4a,7-methano-4aH-naphth[1,8a-b]oxirene

Cas Number:

26619-69-2

Molecular formula:

C15H24O

IUPAC Name:

[1aR-(1aα,4aα,7α,8aS*)]-octahydro-4,4,8,8-tetramethyl-4a,7-methano-4aH-naphth[1,8a-b]oxirene

impurity 1

Reference substance name:

7,7,8,8-tetramethyloctahydro-2,3b-methanocyclopenta[1,3]cyclopropa[1,2]benzen-4-ol

Cas Number:

29461-20-9

Molecular formula:

C15H24O

IUPAC Name:

7,7,8,8-tetramethyloctahydro-2,3b-methanocyclopenta[1,3]cyclopropa[1,2]benzen-4-ol

Test material form:

liquid

Details on test material:

- Name of test material (as cited in the expert statement): Folenox
- Chemical name: 4,4,8,8-Tetramethyloctahydro-4a,7-methanonaphtho[1,8a-b]oxirene (IUPAC)
- Empirical formula: C15H24O
- Structural formula: See IUCLID section 1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling for analysis of test concentrations was done at 0h and 48h.

Test solutions

Vehicle:

no

Details on test solutions:

The study started with a combined limit/range-finding test. Twenty daphnids per concentration (four replicates, five daphnids per replicate) were exposed to a control and a WAF at 100 mg/L.
For the final test, test concentrations were WAFs at 0.10, 0.22, 0.46, 1.0, 2.2, 4.6 and 10 mg/L.
Samples taken from the WAFs at 1.0, 2.2, 4.6 and 10 mg/L were analyzed and showed measured initial concentrations of 0.68, 1.6, 3.1 and 7.3 mg/L, respectively. These concentrations remained stable during the test period and were 95-98% of initial at the end of the test. Given these results, the EC50 was calculated using the measured initial concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

180 mg/L expressed as CaCO3

Test temperature:

The temperature was continuously measured in a temperature control vessel. Temperature varied between 19 and 20°C during the test.

pH:

pH 7.7 +/- 0.3

Nominal and measured concentrations:

Nominal concentrations: WAFs at 0.10, 0.22, 0.46, 1.0, 2.2, 4.6 and 10 mg/L.
Measured initial concentrations: 0.68, 1.6, 3.1 and 7.3 mg/L (corresponding to WAFs at 1.0, 2.2, 4.6 and 10 mg/L, respectively).

Reference substance (positive control):

no

Remarks:

Negative control was used - test medium without test item or other additives. Reference test carried out 14-16 Nov 2016 to check sensitivity of test system used by the laboratory.

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.8 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The 48h-EC₅₀was 1.8 mg/L based on measured initial concentrations (95% confidence interval between 1.6 and 2.7 mg/L).