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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Modifying effects of components of plant essence on the induction of sister-chromatid exchanges in cultured Chinese hamster ovary cells
Author:
Sasaki YS, et al.
Year:
1989
Bibliographic source:
Mutation Research, 226, 103-110

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 479 (Genetic Toxicology: In Vitro Sister Chromatid Exchange Assay in Mammalian Cells)
Principles of method if other than guideline:
In principle the method descibed in OECD Guideline 479 was used with some deviations. Test substance was only investigated in absence of an appropriate metabolic system.
GLP compliance:
not specified
Type of assay:
sister chromatid exchange assay in mammalian cells

Test material

Constituent 1
Chemical structure
Reference substance name:
Linalool
EC Number:
201-134-4
EC Name:
Linalool
Cas Number:
78-70-6
Molecular formula:
C10H18O
IUPAC Name:
3,7-dimethylocta-1,6-dien-3-ol
Details on test material:
- Name of test material (as cited in study report): Linalool

Method

Species / strain
Species / strain / cell type:
Chinese hamster Ovary (CHO)
Metabolic activation:
without
Test concentrations with justification for top dose:
0, 33.3, 100, 333, 1000 µM
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO
Controls
Untreated negative controls:
other: not specified if solvent or negative control
Negative solvent / vehicle controls:
other: not specified if solvent or negative control
True negative controls:
no
Positive controls:
yes
Positive control substance:
mitomycin C
Details on test system and experimental conditions:
METHOD OF APPLICATION: in medium

DURATION
- Preincubation period: none
- Exposure duration: 21 hours

SPINDLE INHIBITOR (cytogenetic assays): colchicine

NUMBER OF CELLS EVALUATED: 50 per culture

Results and discussion

Test results
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
other: unclear if solvent or negative control
Untreated negative controls validity:
not examined
Positive controls validity:
valid
Remarks on result:
other: other: CHO K-1 cells
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Component of plant essence

MMC treatment (0.15µM)

Mean frequency of SCEs

Concentration  (µM) of components

0

33.3

100

333

1000

Linalool

+

81.7

83.4

81.7

80.2

77.1

-

9.0

9.0

8.9

8.8

9.0

Applicant's summary and conclusion