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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD Guideline and following the priciples of Good Laboratory Practice

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea
EC Number:
813-604-1
Cas Number:
68533-01-7
Molecular formula:
C8 H16 N4 O2
IUPAC Name:
[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
batch number: 492-14-01.
the purity is 99.08 area % (190 nm) and 98.17 area % (200 nm)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The test organism, Daphnia magna Straus originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch
Pszczyna, Department of Ecotoxicology[SOP/W/67]. Only organisms up to 24 hours old (not first brood progeny) and in good physiological
condition were used in the tests.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19.1 – 20.4ºC
pH:
7.28 – 7.30
Dissolved oxygen:
9.00 – 9.01 mg/L (initial)
8.75 – 8.76 mg/L (final)
Nominal and measured concentrations:
120 mg/L, plus the control
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The LOEC/48 h is higher than the test item concentration of 120 mg/L.
The NOEC/48 h is higher than or equal to the test item concentration of 120 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes