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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD Guideline and following the priciples of Good Laboratory Practice

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea
EC Number:
813-604-1
Cas Number:
68533-01-7
Molecular formula:
C8 H16 N4 O2
IUPAC Name:
[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
NM01; batch no.: 492-14-01
Purity: 99.08 area % (190 nm) and 98.17 area % (200 nm)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
In the definitive test following test item concentrations were used: 31.3, 62.5, 125, 250 and 500 mg/L
plus the control (with a spacing factor of 2.0).

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22.0 – 22.4°C
pH:
The pH values were in the ranges of 7.14 – 7.28 at exposure initiation and 8.11 – 8.58 at exposure termination
Nominal and measured concentrations:
In the definitive test following test item concentrations were used: 31.3, 62.5, 125, 250 and 500 mg/L
plus the control (with a spacing factor of 2.0).
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

The EyC50/72 h is higher than the test item concentration of 500 mg/L.

The EyC10/72 h is 264 mg/L (95% confidence limits were not determined).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes