Reaction mass of 1,5-dihydroxy-4-nitro-8-(phenylamino)anthraquinone and 1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Test substance: Terasil Blau GRN Isomerengemisch roh feucht
Code: FAT 92504/A
Batch-No.: 1/95
Appearance: solid blue
Solubility (g/l): <0.1
Storage: room temperature
Expiration date: 04/1999

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

None

Test organisms (species):

Daphnia magna

Details on test organisms:

None

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21 °C

pH:

7.6 - 7.8

Dissolved oxygen:

97 - 99 % at the end of the exposure

Nominal and measured concentrations:

0.1, 1, 10 and 100 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

1 out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48-hour exposure period. Apart from this, no mortality was observed at any other cocentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups.

Results with reference substance (positive control):

None

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.

Executive summary:

The short term toxicity of FAT 92504/A to aquatic invertebrates was evaluated in a study conducted according to OECD Guideline 202. In this study, groups of 10 daphnids were exposed to the test concentrations of 0.1, 1, 10 and 100 mg/L for 48 hours. The daphnids were also exposed to blank and vehicle groups. The observations of mobility were recorded to determine the toxicity of the substance. 1 out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48-hour exposure period. Apart from this, no mortality was observed at any other concentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups. Hence, the 48h EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.

Description of key information

The short term toxicity of FAT 92504/A to aquatic invertebrates was evaluated in a study conducted according to OECD Guideline 202. In this study, groups of 10 daphnids were exposed to the test concentrations of 0.1, 1, 10 and 100 mg/L for 48 hours. The daphnids were also exposed to blank and vehicle groups. The observations of mobility were recorded to determine the toxicity of the substance. One out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48-hour exposure period. Apart from this, no mortality was observed at any other concentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups. Hence, the 48-hour EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L