Feldspar minerals, hematite and quartz, calcination products of copper mining residues

Bioaccumulation: terrestrial

Administrative data

Endpoint:

bioaccumulation: terrestrial

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific standards, well documented and acceptable for hazard assessment

Data source

Reference

Reference Type:

publication

Title:

Metal accumulation strategies in saprophagous and phytophagous soil invertebrates: a quantitative comparison.

Author:

Gräff S, Berkus M, Alberti G, Köhler HR

Year:

1997

Bibliographic source:

Biometals 10(1): 45-53.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Calculation of BSAF values using terrestrial isopods which were exposed for 3 weeks to lead containing decomposed leaf litter.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Lead dichloride

EC Number:

231-845-5

EC Name:

Lead dichloride

Cas Number:

7758-95-4

Constituent 2

Reference substance name:

007758-95-4

Cas Number:

007758-95-4

IUPAC Name:

007758-95-4

Details on test material:

No further information on test material is stated.

Radiolabelling:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Porcellus scaber

Details on test organisms:

Adults of Porcellio saber were supplied from a laboratory hatchery.

Test conditions

Test temperature:

15°C

pH:

not reported

TOC:

not reported

Moisture:

not reported

Details on test conditions:

-laboratory experiment
- Organisms were exposed to partly decomposed leaf litter material soaked in an aqueous solution of 100, 500 or 1000mg/kg Pb2+ (as PbCl2), or 500, 1000 for three weeks. Controls were wetted with tap water. Isopods were kept in plastic boxes on a moist gypsum base covered by the above mentioned partly decomposed leaf litter material; To adjust exposure conditions to the respective preferences of the test species and to meet the field situation.

Nominal and measured concentrations:

measured lead concentrations (mg Pb/kg dw): 7.1 (control), 517.1, 2777.5 and 7676.0

Results and discussion

Bioconcentration factor

Bioconcentration factor 1

Type:

BSAF

Value:

0.41 other: kg dw / kg dw

Basis:

whole body d.w.

Calculation basis:

steady state

Remarks on result:

other: duration: 21 days; organism: Porcellio scaber; substrate: control

Bioconcentration factor 2

Type:

BSAF

Value:

0.14 other: kg dw / kg dw

Basis:

whole body d.w.

Calculation basis:

steady state

Remarks on result:

other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 100 mg Pb/L

Bioconcentration factor 3

Type:

BSAF

Value:

0.08 other: kg dw / kg dw

Basis:

whole body d.w.

Calculation basis:

steady state

Remarks on result:

other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 500 mg Pb/L

Bioconcentration factor 4

Type:

BSAF

Value:

0.03 other: kg dw / kg dw

Basis:

whole body d.w.

Calculation basis:

steady state

Remarks on result:

other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 1000 mg Pb/L

Reported statistics:

Student's t-test; p<0.001 (higly significant)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Results can be used in a weight of evidence approach to calculate bioaccumulation factors in soil.