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Environmental fate & pathways

Bioaccumulation: terrestrial

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Administrative data

Endpoint:
bioaccumulation: terrestrial
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets generally accepted scientific standards, well documented and acceptable for hazard assessment

Data source

Reference
Reference Type:
publication
Title:
Metal accumulation strategies in saprophagous and phytophagous soil invertebrates: a quantitative comparison.
Author:
Gräff S, Berkus M, Alberti G, Köhler HR
Year:
1997
Bibliographic source:
Biometals 10(1): 45-53.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Calculation of BSAF values using terrestrial isopods which were exposed for 3 weeks to lead containing decomposed leaf litter.
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Lead dichloride
EC Number:
231-845-5
EC Name:
Lead dichloride
Cas Number:
7758-95-4
Constituent 2
Reference substance name:
007758-95-4
Cas Number:
007758-95-4
IUPAC Name:
007758-95-4
Details on test material:
No further information on test material is stated.
Radiolabelling:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Porcellus scaber
Details on test organisms:
Adults of Porcellio saber were supplied from a laboratory hatchery.

Test conditions

Test temperature:
15°C
pH:
not reported
TOC:
not reported
Moisture:
not reported
Details on test conditions:
-laboratory experiment
- Organisms were exposed to partly decomposed leaf litter material soaked in an aqueous solution of 100, 500 or 1000mg/kg Pb2+ (as PbCl2), or 500, 1000 for three weeks. Controls were wetted with tap water. Isopods were kept in plastic boxes on a moist gypsum base covered by the above mentioned partly decomposed leaf litter material; To adjust exposure conditions to the respective preferences of the test species and to meet the field situation.



Nominal and measured concentrations:
measured lead concentrations (mg Pb/kg dw): 7.1 (control), 517.1, 2777.5 and 7676.0

Results and discussion

Bioconcentration factoropen allclose all
Type:
BSAF
Value:
0.41 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: control
Type:
BSAF
Value:
0.14 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 100 mg Pb/L
Type:
BSAF
Value:
0.08 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 500 mg Pb/L
Type:
BSAF
Value:
0.03 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 1000 mg Pb/L
Reported statistics:
Student's t-test; p<0.001 (higly significant)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Results can be used in a weight of evidence approach to calculate bioaccumulation factors in soil.

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