4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21-03-2017 - 25-03-2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Specific details on test material used for the study:

Test Item Name 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride
CAS No. 6358-36-7
Batch No. KCP/FS/44/17
Physical Appearance Yellow powder
Date of manufacturing 2017-05-01
Date of expiry 2017-04-12
Purity not avaiable in COA
Stability Stable in recommended storage conditions
Storage Conditions 1. Ambient Temp (23 to 27 °C) (Keep container tightly closed in a dry & well-ventilated place. Store in cool place. Do not expose to air, light &
moisture.)
Precautions Wear respiratory protection. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. Avoid breathing dust.
Solubility in Water 1531.4 mg/L

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 1 hour continuous stirring. After that analytically detect stock,solubility found 981.56 mg/L and calculations done accordingly. Concentrations decided as follows 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source:Koyal aquarium, Nagpur
- Age at study initiation (mean and range, SD): -
- Length at study initiation (length definition, mean, range and SD):average 1.79 cm
- Weight at study initiation (mean and range, SD):0.1204g (average)
- Method of breeding: -
- Feeding during test: -
- Food type:Standard brand feed,
- Amount: -
- Frequency: once daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.03

Dissolved oxygen:

6.41 mg/L

Salinity:

-

Conductivity:

-

Nominal and measured concentrations:

0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L

Details on test conditions:

Photoperiod: 16 hours light and 8 hours dark
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates: Test started at : 13-03-2017
Test Exposure date: 21-03-2017
Date of experimental termination : 25-03-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.625 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
0.625	4	4	-	-	-	-	-	2	2	2	2	2	2	2
1.25	4	4	-	-	-	-	-	2	4	4	4	4	4	4
2.5	4	4	-	-	-	-	4	4	4	4	4	4	4	4
5	4	4	4	4	4	4	4	4	4	4	4	4	4	4
10	4	4	4	4	4	4	4	4	4	4	4	4	4	4

 

Visible Symptoms:

Nominal Concentration (mg/l)	Number of fish		Symptom (cumulated) after
			3 h		6 h		24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	NS4	NS4	NS4	NS4	NS4	NS4	NS4	NS4	NS4	NS4	NS4	NS4
0.625	4	4	M4	M4	M4	M4	M4	M4	M2	M2	M2	M2	M2	M2
1.25	4	4	M4	M4	M4	M4	M4	M4	-	-	-	-	-	-
2.5	4	4	IS4	IS4	IS4	-	-	-	-	-	-	-	-	-
5	4	4	IS4	IS4	-	-	-	-	-	-	-	-	-	-
10	4	4	IS4	IS4	-	-	-	-	-	-	-	-	-	-

 

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

  

pH and oxygen content:

Nominal Concentration (mg/l)	Number of fish		pH readings after
			24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	8.04	8.04	8.12	8.15	8.23	8.25	8.32	8.35
0.625	4	4	8.13	8.15	8.01	8.02	8.23	8.25	8.36	8.30
1.25	4	4	8.21	8.22	-	-	-	-	-	-
2.5	4	4	-	-	-	-	-	-	-	-
5	4	4	-	-	-	-	-	-	-	-
10	4	4	-	-	-	-	-	-	-	-

 

Nominal Concentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.22	7.21	7.01	7.01	6.90	6.90	6.80	6.82
0.625	4	4	7.08	7.09	6.85	6.85	6.51	6.52	6.41	6.41
1.25	4	4	7.05	7.04	-	-	-	-	-	-
2.5	4	4	-	-	-	-	-	-	-	-
5	4	4	-	-	-	-	-	-	-	-
10	4	4	-	-	-	-	-	-	-	-

 

Test temperature:

Nominal Concentration (mg/l)	Number of fish		Temperature (°C) after
			24 h		48 h		72 h		96 h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	22.9	22.9	22.5	22.5	23.2	23.2	22.9	22.9
0.625	4	4	23.5	23.5	22.6	22.6	23.2	23.2	22.9	22.9
1.25	4	4	23.4	23.4	-	-	-	-	-	-
2.5	4	4	-	-	-	-	-	-	-	-
5	4	4	-	-	-	-	-	-	-	-
10	4	4	-	-	-	-	-	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 6358-36-7 on Zebra fish(Danio rerio)was found to be 0.625 mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) 4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride The nominal concentration selected for the experiment were 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L and test fish were exposed to these concentration for 96 hours. The lethal

concentrations LC50 was found to be 0.625 mg/L.

LC50 (96 hours) Experimental = 0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 6358-36-7 on Zebra fish (Danio rerio) was found to be 0.625 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.625 mg/L

Additional information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride. The nominal concentration selected for the experiment were 0.625mg/L,1.25mg/L,2.5mg/L,5mg/L,10mg/L and test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be 0.625 mg/L.

LC50 (96 hours) Experimental = 0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 1.25 mg/L

Thus considering the above value, it can be concluded that 4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride exhibits toxicity to fish in the acute 1 classification category. Also since long term toxicity data is not available and the substance is inherently biodegradable (near to not readily biodegradable), the final classification for fish is considered to be Aquatic Acute 1 as well as Chronic category 1 as per CLP criteria.