Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
None
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
not specified
Details on test solutions:
None
Test organisms (species):
activated sludge
Details on inoculum:
None
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
None
pH:
None
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
100.0, 32.0 and 10.0 mg/l
Details on test conditions:
None
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
None
Results with reference substance (positive control):
The IC50 of reference substance (3,5-Dichlorophenol) = 13 mg/l.
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
not specified
Conclusions:
The IC50 for FAT 40032/D was found to be greater than 400 mg/L.
Executive summary:

A key toxicity study of FAT 40032/D to activated sludge was determined in a 3-hours respiration inhibition test following methodology similar to OECD Guideline 209. At 10, 32 and 100 mg/L concentrations, respiratory inhibitions observed were 0, 6.3 and 15.8 %, respectively. Based on the findings of the study, it was concluded that the IC50 (3 h) of the test substance FAT 40032/D is greater than 400 mg/L.

Description of key information

The IC50 for FAT 40032/D was found to be greater than 400 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
400 mg/L

Additional information

The IC50 for Reactive Brown 11 was reported to be >300 mg/L in the four available study reports. In the key toxicity study of FAT 40032/D to activated sludge, conducted according to methodology similar to OECD Guideline 209. In this 3-hours respiration inhibition test, the test substance concentrations tested were 100.0, 32.0 and 10 mg/l. Based on the findings of the study, the IC50 (3 h) of the test substance FAT 40032/D was found to be >400 mg/L.