Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: refer below principle
Principles of method if other than guideline:
The reduction of aromatic acid i.e 3-phenylpropionic acid by the hyperthermophilic organism Pyrococcus furiosus was investigated.
GLP compliance:
not specified
Specific details on test material used for the study:
- IUPAC name: 3-phenylpropanoic acid
- Molecular formula: C9H10O2
- Molecular weight: 150.176 g/mol
- Smiles: c1(CCC(O)=O)ccccc1
- InChI: 1S/C9H10O2/c10-9(11)7-6-8-4-2-1-3-5-8/h1-5H,6-7H2,(H,10,11)
- Substance type: Organic
- Physical state: solid
Analytical monitoring:
yes
Details on sampling:
The substrate concentration was 1 mM before inoculation.
Vehicle:
not specified
Test organisms (species):
other: Pyrococcus furiosus
Details on inoculum:
P. furiosus (DSM 3638) was grown anaerobically at 90°C in a basic medium.Yeast extract, cysteine, vitamins, and trace elements were added a Potato starch (5 g/1) was used as a carbon and energy source. Cells were grown in 100 ml bottles containing 50 ml medium,which was flushed with N2/CO2 (80/20) before inoculation. During growth, the bottles were shaken continuously at 200 rev./min. The medium was inoculated with a 2% preculture.
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
25 h
Remarks on exposure duration:
experimental conditions occured for 18 - 25 h after
Hardness:
No data
Test temperature:
90 deg.C
pH:
6.8-7.0
Dissolved oxygen:
No data
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
1 mM which is equivalent to 150.1 mg/l
Details on test conditions:
The start of growth the pH was 6.8 - 7.0 and was not adjusted during the experiment.
Reference substance (positive control):
not specified
Key result
Duration:
25 h
Dose descriptor:
EC50
Effect conc.:
150.1 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: In batch conversions 3-phenylpropionic acid was reduced with yield 69%.

Yield of alcohols from aromatic acid i.e 3-phenylpropanoic acid reduced byP. furiosus in batch culture.

Substrate

Product

Yield (%)

3-Phenylpropionic

acid

3-Phenyl-1-propanol

68.7 ±4.2

 

Validity criteria fulfilled:
not specified
Conclusions:
In batch conversions experiment the test chemical 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.
Executive summary:

The reduction of aromatic acid i.e 3-phenylpropionic acid by the hyperthermophilic organism Pyrococcus furiosus was investigated.

P. furiosus (DSM 3638) was grown anaerobically at 90°C in a basic medium.Yeast extract, cysteine, vitamins, and trace elements were added a Potato starch (5 g/1) was used as a carbon and energy source. Cells were grown in 100 ml bottles containing 50 ml medium,which was flushed with N2/CO2 (80/20) before inoculation. During growth, the bottles were shaken continuously at 200 rev./min. The medium was inoculated with a 2% preculture.

Analytical method used as HPLC on a Nucleosil 100 C18 5U(Alltech, Laarne, Belgium) column. A Waters 600 controller and pump and a Waters 996 photodiode array detector were used (all Millipore, Milford, USA).Methanol/1% acetic acid was used as eluent and the analysis was isocratic.

1mM substrate concentration was used in experiment which is equivalent to 150.1 mg/l.

In batch conversions 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.

Description of key information

In batch conversions experiment the test chemical 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
150.1 mg/L

Additional information

The reduction of aromatic acid i.e 3-phenylpropionic acid by the hyperthermophilic organism Pyrococcus furiosus was investigated.

P. furiosus (DSM 3638) was grown anaerobically at 90°C in a basic medium.Yeast extract, cysteine, vitamins, and trace elements were added a Potato starch (5 g/1) was used as a carbon and energy source. Cells were grown in 100 ml bottles containing 50 ml medium,which was flushed with N2/CO2 (80/20) before inoculation. During growth, the bottles were shaken continuously at 200 rev./min. The medium was inoculated with a 2% preculture.

Analytical method used as HPLC on a Nucleosil 100 C18 5U(Alltech, Laarne, Belgium) column. A Waters 600 controller and pump and a Waters 996 photodiode array detector were used (all Millipore, Milford, USA).Methanol/1% acetic acid was used as eluent and the analysis was isocratic.

1mM substrate concentration was used in experiment which is equivalent to 150.1 mg/l.

In batch conversions 3-phenylpropionic acid was reduced with the highest yield (69%) with Substrate concentrations 1 mM which is equivalent to 150.1 mg/l.