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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
basic toxicokinetics
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication.

Data source

Reference
Reference Type:
publication
Title:
The toxic and metabolic effects of 23 aliphatic alcohols in the isolated perfused rat liver
Author:
Strubelt O, Deters M, Siegers C-P and Younes M
Year:
1999
Bibliographic source:
J. Toxicological Sci. 49: 133-142

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The objective of this study was to evaluate the relationship between chemical structure and hepatic efficiacy of the test materials. The toxic, functional, and metabolic effects of several test materials in the perfused, isolated rat liver were investigated.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylbut-2-en-1-ol
EC Number:
209-141-4
EC Name:
3-methylbut-2-en-1-ol
Cas Number:
556-82-1
Molecular formula:
C5H10O
IUPAC Name:
3-methylbut-2-en-1-ol
Details on test material:
- Name of test substance: 3-Methyl-2-buten-1-ol
- Source: Merck-Schuchardt
- Analytical purity: analytical grade, no further data on purity
Radiolabelling:
no

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Weight at study initiation: 320-380 g
- Diet: Altromin pellets standard diet, ad libitum
- Water: tap water, ad libitum

Administration / exposure

Route of administration:
other: via the perfusate
Vehicle:
not specified
Details on exposure:
Liver perfusion:
Removal of the liver and its connection to a recirculation perfusion system was performed. Livers were weighed before they were connected to the perfusion system. The albumin- and serum-free perfusion medium consisted of 250 ml Krebs-Henseleit buffer, ph 7.4; additionally sodium laurocholate (36.7 g/l) was infused into the perfusate at a rate of 1.2 ml/h to stimulate bile secretion. The perfusion medium was continuously gased with carbogen yielding an oxygen partial pressure of 600 mm Hg and kept at 34 °C.
Perfusion was performed under conditions of constant pressure (240 mm H2O) throughout the experiment. The perfusion flow rate was 60 ml/min and the experiments were started after a 30 min equilibration period, adding the test material to the perfusate (time 0) and finished 120 min after initiation. The oxygen consumption was calculated from the difference in oxygen concentrations of the influent and the efluent perfusate using a Micro pH/Blood Gas Analyzer 1306 (Instrumentation Laboratory). The perfusion flow rate was determined every 30 min, bile was sampled every 30 min and the rate of bile secretion was calculated per gram liver and minute.
For biochemical determinations, samples of 2 ml were also taken from the perfusate every 30 min. After the experiments the livers were frozen in liquid nitrogen for further analysis.
Duration and frequency of treatment / exposure:
120 min
Doses / concentrations
Remarks:
Doses / Concentrations:
65.1 mmol/l
No. of animals per sex per dose / concentration:
not applicable
Control animals:
other: control experiments were performed in the same way, adding 0.9% saline instead of the test material to the perfusate.
Details on study design:
Biochemical determinations:
The activities of glutamate-pyruvate-transaminase (GPT), lactate dehydrogenase (LDH) and glutamate dehydrogenase (GLDH), as well as the concentrations of lactate and pyruvate in the perfusate were assayed using commercial kits from Boehringer, Mannheim. Malondialdehyde (MDA) was measured in the perfusate and in the livers by coupling to thiobarbituric acid. Total glutathione was determined in liver and perfusate samples, oxidized glutathione (GSSG) was estimated after blocking reduced glutathione (GSH) with 2-vinylpyridine. For ATP determination, hepatic tissue was frozen immediately in liquid nitrogen and exracts were prepared. ATP was assayed enzymatically using a reagent kit from Sigma (Munich).
Statistics:
Means +/- SEM were calculated using the usual manner. The difference between 2 means was calculated using Dunnet´s t-test setting p = 0.05 as the limit of significance. Correlation coefficients were calculated using the program Sigma Plot 3.0 (Jandel Scientific). Their significance was evaluated according to tables published in Documenta Geigy (1969).

Results and discussion

Applicant's summary and conclusion